| Objective:1.Prepare g-C3N4 emitted with long wavelengths and apply it to biological imaging to eliminate biological self-fluorescence interference,so as to achieve multi-channel imaging.2.Synthesize g-C3N4 with a larger specific surface area and apply it to drug delivery,deliver complexes into cells,and study its anti-tumor activity in vitro.Methods:1.The modified three-stage program heating method was used to prepare the structuretwisted g-C3N4,which activated more n-π*transitions in the chemical structure;At the same time,in view of the problem that the material is not water-soluble and cannot act on cells for imaging,strong acid oxidation and ultrasonic treatment are used to prepare solid samples into colloidal solutions.2.g-C3N4 with a larger specific surface area was prepared by supramolecular selfassembly and applied to the field of drug delivery,using the anticancer drug doxorubicin as a drug model to discuss its drug loading,sustained release and cell uptake at different pH value.Results:1.With melamine as the only precursor,Tx-MACN powder was prepared by programcontrolled temperature strategy.Through subsequent oxidation and sonication,we obtained a yellow T675-MACN colloid with good biosafety and water solubility,no significant aggregation,and fluorescence stable for up to 10 months.The T675-MACN colloid excites green fluorescence in the dark with a wide fluorescence emission band centered at 508 nm.With ZEISS observations,samples can be efficiently imaged in the blue,green,and red channels.2.The drug carrier prepared by self-assembly has a drug loading efficiency of 27%at pH 5,low toxicity and can be effectively taken up by cancer cells,with pH-responsive drug loading and release characteristics,and showing significant in vitro anti-tumor activity.Conclusion:1.T675-MACN colloidal sample can realize multi-channel imaging and can be used as an efficient fluorescent developer.2.CA-MA2 can be used as an effective vehicle for drug delivery. |
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