| Supramolecular chemistry is also known as host-guest chemistry.It is the product of the integration of many disciplines such as chemistry,physics,material science,information science,and environmental science.The main research content of supramolecular chemistry is the connection of multiple chemical components based on covalent interactions.As one of the main macrocyclic compounds,cyclodextrin has the characteristics of stability,low toxicity,and easy structural modification.Therefore,it is widely used in many fields such as medicine,analysis,catalysis,biological fluorescence sensing,and imaging.After binding to a variety of fluorophores,cyclodextrins can be used as fluorescent probes for the specific and selective sensing of drugs,ions,biomolecules,and other organic compounds in the field of chemical analysis.In this paper,a fluorescent sensor was constructed by using macrocyclic compound cyclodextrin.Additionally,the fluorescence sensor was used to detect amantadine and p-nitrophenol.The main contents of this paper are as follows.1.Fluorescent cyclodextrins are recognized mainly by using the basic pattern of fluorophore inclusion-guest ejection.This kind of fluorescent cyclodextrin sensor usually uses the cyclodextrin cavity itself as the recognition site and it relies on the hydrophobicity and size of the cavity for recognition.This work designed and prepared a half cyanine modified cyclodextrin dyes 4-[4-(dimethylamino)styryl]-1-β-cyclodextrin pyridine p-toluenesulfonate(DASP-β-CD)as a probe for amantadine(AMD)fluorescence detection.The fluorescence excitation and emission properties of DASP-β-CD were characterized by fluorescence spectroscopy.According to FT-IR and ~1H NMR results,host-guest inclusion occurred after amantadine was added to DASP-β-CD aqueous solution.The resulting AMD@DASP-β-CD host-guest inclusion products destroyed the intermolecular inclusion of DASP-β-CD,and amantadine entered the cavity of DASP-β-CD,which resulted in weakened fluorescence emission.In order to further reduce the fluorescence emission,the effects of solvent p H and temperature on the fluorescence emission of DASP-β-CD were investigated.The results showed that the optimum experimental conditions were p H=6 and temperature 20°C.Based on the above situation,we developed a fluorescence analysis method for the detection of amantadine.2.A functional fluorescent probe 4-[4-(acetylamino)styryl]-1-cyclodextrin pyridine p-toluenesulfonate(ASPT-β-CD)for 4-NP detection was designed by combining hemicyanine dye withβ-CD.4-NP is a common pollutant in water.In this section,we studied its optical properties and found that the fluorescence excitation spectrum of ASPT-β-CD has a large overlap area with the ultraviolet-visible absorption spectrum of 4-NP.Therefore,a method for the detection of 4-NP by internal filtering effect was designed.After the addition of p-nitrophenol,it entered the cyclodextrin cavity of ASPT-β-CD,and fluorescence of ASPT-β-CD was quenched due to IFE.The effects of polar solvents and p H on the IFE were investigated.It was found that the effect of IFE reached the maximum when p H=8 in water solvent.A fluorescence analysis method was established based on above results for the detection of 4-NP with high sensitivity and selectivity.The results showed that the 4-NP@ASPT-β-CD host-guest inclusion system exhibited sensitive performance of fluorescence sensing for 4-NP. |
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