Fabrication And Characteristics Research Of Albumin-based Antibacterial Hydrogel

Bacterial infections constitute one of the greatest challenges facing public healthcare,posing a great harm to human health.Hydrogel is a kind of three-dimensional network polymer material formed by physically or chemically cross-linked chains and has been widely applied in biomedical field as an ideal biomaterial due to their high hydrophilicity,high drug loading capacity and adjustable mechanical properties.Hydrogels synthesized from natural macromolecular protein possess better biological security and function ability than that formed by synthetic polymers.In this thesis,two self-derived albumin-based hydrogels were constructed for antibacterial application and drug delivery,which exhibited excellent biocompatibility and broad-spectrum antibacterial performance,and will have wide potential clinical applications.The main research contents are as follows:（1）A dynamic double-crosslinked albumin-based antibacterial hydrogel was designed and constructed by innovatively introducing tetramethylphosphorous sulfate（THPS）,a green bactericide,into bovine serum albumin/glutathione（BSA/GSH）matrix.The skeleton of hydrogels was cross-linked by reversible disulfide bonds and THPS-NH₂ chemical bonds.While forming a stable gel network,THPS exerted 99.9%broad-spectrum and excellent antibacterial effect on E.coli and S.aureus through being sustainable released from the hydrogel.The BSA/GSH-THPS antibacterial hydrogel also possesses good biocompatibility and self-healing properties.This albumin-based hydrogel with low cost is expected to realize wide biomedical applications.（2）Similarly,bovine serum albumin（BSA）was used as the hydrogel matrix to construct a disulfide cross-linked network based on redox reaction.The oxidation and solvent property of dimethyl sulphoxide（DMSO）was combined and introduced into the fabrication of albumin-based hydrogels with narrow-needle injection and self-healing properties.The redox hydrogel realized the in-situ loading of dihydromyricetin（DMY）,indicating the hydrogel can be used as an ideal drug carrier for DMY.The hydrogel also exhibited low cytotoxicity and good biodegradability,which can be used as an injectable implant.It is a new functional development for albumin as well as providing a feasible strategy in increasing the bioavailability of DMY,which has potential application value.