Synthesis And Properties Of Organic Fluorescent Compounds Based On Phenothiazine

In recent years,fluorescent probes for the detection of metal ions,cyanide ions,and peroxynitrite anions and new photosensitizers for photodynamic therapy have been developed one after another.However,due to the poor selectivity and polymerization-induced inhibition effect of these fluorescent probes,the new photosensitizers have problems such as low singlet oxygen generation rate,which are difficult to be widely used.Therefore,the development of fluorescent probes with simple detection,good selectivity and high sensitivity and photosensitizers with high singlet oxygen generation rate are still the focus of research.In this paper,phenothiazine was used as the parent,and five target products were designed and synthesized,and their properties were studied:（1）Synthesize the target product M₁ with dual effects of fluorescent probe and photosensitizer.In the spectral test,it was found that M₁ can specifically recognize CN^-,with good selectivity and strong anti-interference,and the detection limit is 4.9×10^-4 mol/L;at the same time,the singlet oxygen yield of M₁ is higher than that of Rose Bengal,it is an excellent photosensitizer,and it is expected to play a role in photodynamic therapy.（2）The synthetic target product M₂ has a fluorescence effect on the ONOO^-solution after spectral testing,the fluorescence intensity at the maximum emission wavelength is significantly reduced,and the maximum emission wavelength undergoes a blue shift,and the intensity is weakened,which indicates that the probe molecule M₂ can specifically recognizes ONOO^-with a detection limit of 1.2×10^-3 mol/L.（3）The synthesized target product M₃ shows excellent fluorescence properties in the detection of Ag⁺and Hg²⁺,and can identify Ag⁺and Hg²⁺at the same time.The detection limits are 1.9×10^-4 mol/L and 2.8×10^-4 mol/L,respectively,can be used for the detection of trace heavy metal ions Ag⁺and Hg²⁺in organisms,and has great potential in subsequent research and development.（4）In addition,the target products M₄ and M₅ were synthesized,and their fluorescence recognition properties for various ions were studied.