Antibacterial Activities Of Fe₃O₄/CuO_x Nanozyme

Bacterial infections are a major problem in global public health.Despite great success in the use of antibiotics against bacterial infectious diseases,the increasing number of antibiotic-resistant strains,especially multidrug-resistant（MDR）species,greatly reduces the effectiveness of antibiotic treatment and leads to high mortality rates.Developing new antibacterial materials is an effective way to solve MDR bacterial infection.Among them,nano-simulated enzyme is a new antibacterial material,because of its remarkable catalytic activity,stability and low cost.They were developed to address the limitations of natural and conventional artificial enzymes.With the great progress of nanotechnology,biotechnology,catalytic science and computing technology,nanozymes have been widely used in many fields such as disease treatment,biological detection and environmental bacteriostasis.In this study,Fe₃O₄/CuO_x composite with high peroxidase-like activity and excellent antibacterial properties was prepared.The morphology and structure of Fe₃O₄/CuO_x composite and the catalytic activity of simulated enzyme were determined.The effects of material concentration,temperature and p H on the catalytic activity were investigated.To investigate the antibacterial effect of Fe₃O₄/CuO_x composite on two bacteria（S.aureus and E.coli）.The experimental results are as follows:1.Fe₃O₄/CuO_x composites were prepared to obtain Fe₃O₄/CuO_x composites,which have high peroxidase catalytic activity and are capable of generating large amounts of ROS.2.The Fe₃O₄/CuO_x composite can effectively kill E.coli and S.aureus.In addition,Fe₃O₄/CuO_x is superparamagnetic,can be recycled by external magnetic fields and still has a good antibacterial effect.3.Fe₃O₄/CuO_x-based band-aids were prepared and used for anti-infection treatment.The results showed that the Fe₃O₄/CuO_x composite could significantly inhibit the growth of bacteria in the wounds of mice and promote wound healing.In conclusion,a novel Fe₃O₄/CuO_x nanoantibacterial material has been developed,which can be effectively used in the fields of antibacterial and anti-infection.This study will provide a theoretical basis and practical foundation for the preparation of nano-mimetic enzymes and their functionalization.