Nano MOF Fluorescent Probes Are Used For The Detection Of Active Molecules In The Process Of Atherosclerosis

Atherosclerosis is a chronic inflammatory cardiovascular disease characterized by the formation of lipid-rich plaques in the walls of large and medium-sized arteries.The current diagnosis method is after plaque formation,through vascular color Doppler ultrasound,angiography and other methods to check,but there are still some challenges in the current diagnosis method for early evaluation of atherosclerotic disease.Active molecules play an important role in maintaining normal physiological activities.When the level of active molecules in the body is abnormal,various diseases will ensue.Among them,protein phosphorylation,cysteine,glutathione and ascorbic acid are almost all involved in the life process and play an important role in the life process.Therefore,it is of great significance to design and develop new analytical methods to explore the correlation between changes in various active molecular levels and diseases during the occurrence and development of atherosclerotic diseases for the early diagnosis and mechanism research of atherosclerotic diseases.Based on the metal-organic framework,two double-detection nanofluorescent probes,UIO-PC and Al-NP-AG,were designed and synthesized for simultaneous fluorescence detection of phosphate and cysteine,glutathione and ascorbic acid,respectively.The probe has been successfully applied to the detection and analysis of active substances in serum in the early stage of atherosclerotic disease.By measuring the changes of serum active molecules in the early stage of the disease,the effect of early diagnosis can be achieved,which lays a foundation for the future study of various signaling pathways and disease mechanisms.Specific work is as follows:1.Using citric acid as carbon source and p-phenylenediamine as nitrogen source,citrate-p-phenylenediamine carbon point was synthesized by hydrothermal method and encapsulated into UIO-MOF.Nano fluorescent probe UIO-PC for double detection of phosphate and cysteine was constructed,thus achieving fluorescence detection of phosphate and cysteine in serum of atherosclerosis and acute hyperuricemia.Using citric acid as carbon source and p-phenylenediamine as nitrogen source,carbon point was synthesized.When excited at 370 nm,cysteine was detected at 510 nm,emitting green fluorescence.Using Zr as the metal active center,the UIO-66-NH₂-H₂TCPP metal-organic framework was synthesized.Due to the specific binding of phosphoric acid to Zr,the red fluorescence of porphyrin could be released at the emission peak of 645 nm when excited at 415 nm to detect phosphoric acid.When the probe detects cysteine and phosphate at the same time,the excitation wavelength and emission wavelength are different,and the emission peaks will not affect each other,and there is no fluorescence interaction.The morphology and components of the probe were characterized by particle size,potential,SEM,TEM,XRD and IR.Subsequently,the probe was applied to the early stage of atherosclerotic disease in rats,and the contents of serum lipids were measured.H&E staining was performed on the inner wall of aortoabdominal artery and major organs to characterize the early stage of atherosclerotic disease.The serum levels of cysteine and phosphate were detected by the probe.The levels of cysteine and phosphate in serum of diseased rats were higher than those of normal rats.At the same time,a model of acute hyperuricemia disease was established,and the contents of serum uric acid,creatinine,urea nitrogen and pyruvic transaminase in rats were measured,and the severity of liver and kidney damage of this disease was evaluated.The probe was also used to detect the levels of cysteine and phosphate in the serum of rats with acute hyperuricemia.The experimental results showed that in the acute hyperuricemia model,the levels of cysteine and phosphate in the serum of rats in the disease group were lower than those in the normal group.By measuring the levels of active molecules in the serum of rats with preatherosclerosis and acute hyperuricemia,the fluorescence nanoprobe can be used to diagnose or evaluate the progression of the disease in the early stage.The fluorescence nanoprobe can provide an effective fluorescence tool for the subsequent study of related active molecular mechanisms and various signaling pathways.2.The MOF complex was synthesized by modifying small molecules 2,3-diaminaphthalene onto Cu²⁺quenched fluorescence Al-MOF,and the nanofluorescent probe Al-NP-AG was constructed to detect glutathione and ascorbic acid,thus realizing the fluorescence detection of glutathione and ascorbic acid in atherosclerotic serum.2,3-diaminaphthalene reacts with ascorbic acid and emits green fluorescence at 540 nm emission peak when excited at 370 nm to detect ascorbic acid.Cu²⁺can quenchthe fluorescence of Al-MOF.When excited at 415 nm,Cu²⁺and glutathione specific coordination,release the fluorescence of porphyrin in Al-MOF at the emission peak of 650 nm,so as to detect glutathione.When the probe was used for double detection of glutathione and ascorbic acid,the position of emission peak did not affect each other and there was no fluorescence interaction.Subsequently,the probe was characterized by particle size,potential,TEM,XRD,IR,etc.The atherosclerotic model was established,and the serum glutathione and ascorbic acid levels of rats with atherosclerotic disease were detected by the probe.The experimental results showed that the serum glutathione and ascorbic acid levels of rats with atherosclerotic disease were higher than those of normal rats.The fluorescence detection method is rapid and simple,which lays a solid foundation for further study of atherosclerotic diseases.