Separation And Safety Toxicological Evaluation Of Alcohol Extract Components From Atractylodes Macrocephala

In this paper,dried Atractylodes matractylodes tablets were used as raw materials and soaked in 75%ethanol with solid-liquid ratio of 1:20(W/V)for 24 h.The ethanol extract with a yield of 85.31±1.36%was obtained.The contents of various chemical components were detected by coloration.The results showed that the contents of total sugar,reducing sugar,flavonoid,protein and uronic acid were 73.35±1.63%,4.2±0.27%,2.1±0.14%,0.6±0.03%and 1.2±0.21%,respectively.This indicates that the alcohol extract of Atractylodes macrocephala has a high content of polysaccharide,and contains almost no protein and aldehyde acids,but a small amount of flavonoids.Adopt solvent extraction,according to the size of the polarity of compounds of extracted by alcohol extraction separation,the SYM phase,EA phase,ZDC phase and water phase(AAMK).0.05±0.01%、2.4±0.04%、5.98±0.13%、85.02±2.61%.The chemical composition of each component was detected by colorimetric method.The antioxidant capacity of EA phase,ZDC phase and AAMK in vitro was investigated,mainly the scavenging capacity of DPPH free radical,hydroxyl free radical(OH-)and ABTS·free radical were measured respectively.In general,the antioxidant capacity of AAMK is relatively strong,which is of significance for further exploration.The composition and structure of AAMK were analyzed.A full-wavelength scan of AAMK revealed that proteins,polypeptides,and nucleic acids were not present in AAMK.The polysaccharide content of AAMK was 90.42±3.76%,and the average molecular weight of AAMK was determined by HPLC to be approximately 2.5 KDa,as a neutral polysaccharide with uniform molecular weight distribution and high purity.Using infrared chromatography,gas chromatography,hyperiodine oxidation,Smith degradation,methylation experiments results found that the monosaccharide composition and molar ratio of AAMK are arabose(Ara):xylose(Xyl):mannose(Man):glucose(Glu):galactose(Gal)=0.9:1.6:0.8:8.7:0.8,the main chain is composed of 1-Linked-Glcp,existence-configuration polysaccharide structure.The SEM map shows that the AAMK surface structure is a sheet structure with holes,which can easily adsorb substances and are bound by the body cells.In order to study the safety toxicity evaluation of AAMK and provide the safety basis for the next development and utilization of AAMK,acute toxicity and subacute toxicity tests of AAMK were carried out.In the acute test,the mortality rate of mice was 0%,and the MTD and LD50 of AAMK were both greater than 10 g/kg·BW,which belonged to non-toxic substance.The subacute toxicity test was carried out,and the changes of body weight,organ index,blood routine,blood biochemical indexes and histopathological sections of the mice were detected and analyzed.There were no significant differences between the above indexes and the blank group,indicating that AAMK was non-toxic in the subacute toxicity test.Combined with the results of acute toxicity test and subacute toxicity test of AAMK on mice,AAMK is a non-toxic substance,which provides a theoretical basis of safety for further development and utilization.By detecting SOD activity,GSH-Px activity and MDA content in liver and spleen of mice,it was found that AAMK could improve the activity of antioxidant enzyme,reduce the level of lipid peroxidation and improve the antioxidant capacity of mice.