Synthesis Composite Of PILs-rGO-Hemin And Its Application In Electrochemical Biosensor

Artificial biomimetic materials are composite materials with natural enzyme activity.Compared with natural enzymes,these materials have the advantages of low production cost,simple immobilization process and strong environmental stability.To date,various materials with enzyme-like biological activity have been developed and applied to the study of electrochemical biosensors.As the activity center of cytochrome,hemoglobin,peroxidase and other important proteins,heme has enzyme-like biological activity and is an excellent artificial biomimetic material.In this paper,hemin was selected as the electroactive center,and the poly（ionic liquids）/reduced graphene oxide/hemin composite（PILs-rGO-Hemin）composites were prepared by the electrostatic interaction between poly（ionic liquids）/reduced graphene oxide（PILs-rGO）composites and hemin,and hydrogen peroxide-enzyme-free sensors were constructed on this basis.Using PILs-rGO-Hemin as the electronic medium,the glucose oxidase electrochemical sensor was constructed by fixing glucose oxidase.The main research contents and results are as follows:1.Firstly,poly（ionic liquids）/reduced graphene oxide composite（PILs-rGO）was prepared by co-reduction.Using the charge properties of ionic liquids,hemin is indirectly combined with reduced graphene oxide by the bridging of ionic liquids to obtain the poly（ionic liquids）/reduced graphene oxide/hemin composite（PILs-rGO-Hemin）.Zeta potential,TEM,UV-vis,FT-IR and other technologies were used to characterize the charge properties,morphology and structure of these two synthetic materials.Based on the PILs-rGO-Hemin material,an enzyme-free sensor PVA/PILs-rGO-Hemin/GCE toward hydrogen peroxide（H₂O₂）was constructed.The detection range was 100-16000μM,the sensitivity was 0.379μAμM^-1cm^-2,and the detection limit was0.0403μM.Moreover,the enzyme-free sensor shows good reproducibility and stability.2.Using the composite PILs-rGO-Hemin as an electronic medium,a glucose oxidase electrochemical biosensor was constructed,which had a wider detection range and higher sensitivity for glucose detection than other graphene-based glucose oxidase electrochemical biosensors,with detection ranges of 0.01-0.05 and 1-30 m M,respectively,and sensitivities of145.93 and 21.19μA m M^-1cm^-2,respectively.In addition,the modified electrode exhibits strong anti-interference and storage stability.The sensor can be applied to the detection of glucose in serum samples.