| Objective:To investigate the physical properties of self-made superparamagnetic iron oxide nanoparticles(SPION)and the stability of superparamagnetic lipiodol(SPLIP)in vitro and its development on DSA and magnetic resonance.Materials and methods: Superparamagnetic iron oxide nanoparticles were prepared pyrolysis.The morphology and magnetic properties of nanoparticles were characterized by transmission electron microscope(TEM),dynamic light scattering particle size analyzer(DLS)and vibrating sample magnetometer(VSM).Superparamagnetic lipiodol(SPION: lipiodol = 1:2,SPION: lipiodol = 1:1,SPION:lipiodol = 2:1)was synthesized by combining superparamagnetic iron oxide nanoparticles with lipiodol.Compare it with lipiodol on the 1st,7th,14 th and 28 th days to observe whether there are color and odor changes,stratification and precipitation.At the same time,DSA and MRI were performed on day 1 and day 28.The changes of DSA development and T2 signal of magnetic resonance on day 1 and day 28 were observed to explore the stability of SPLIP.Results: Superparamagnetic iron oxide was spherical under electron microscope,with uniform particle distribution,particle size of 241.3 nm and zeta potential of-30.6 MV.It has good superparamagnetism and its saturation magnetization is 22.47 EMU / g.During the observation,the color and smell of superparamagnetic iodized oil in different proportions did not change,nor did stratification and precipitation occur.Through visual observation,the DSA fluoroscopy density did not change,and the MRI scanning signal did not change significantly.Conclusion: Superparamagnetic nanoparticles were successfully prepared and different proportions of superparamagnetic lipiodol were synthesized.The general properties of superparamagnetic lipiodol are stable and its in vitro magnetic resonance imaging has good imaging performance.Objective: Making a preliminary exploration about the biosafety of self-made superparamagnetic iron oxide nanoparticles(SPION)and superparamagnetic lipiodol(SPLIP).Materials and methods: Mouse liver parenchymal cells(AML12)and mouse epithelioid fibroblasts(L929)were cultured,and the cytotoxicity of the synthesized superparamagnetic iron oxide and superparamagnetic lipiodol in vitro was verified by MTT method.Results: The self-made superparamagnetic iron oxide and superparamagnetic lipiodol had no effect on the activity of AML12 cells and L929 cells.The survival rates of AML12 cells in low concentration SPION(SPION1),high concentration SPION(SPION2),low concentration SPLIP(SPION-Mix1)and high concentration SPLIP(SPION-Mix2)groups were(97.86 ± 2.48)%,(95.46 ± 5.51)%,(97.14 ±2.90)%,(97.04 ± 2.65)%,respectively.The survival rates of L929 cells in low concentration SPION(SPION1),high concentration SPION(SPION2),low concentration SPLIP(SPION-Mix1)and high concentration SPLIP(SPION-Mix2)groups were(99.56 ± 1.53)%,(100.72 ± 4.83)%,(89.93 ± 8.29)%,(95.07 ± 4.35)%,respectively.Conclusion: The self-made superparamagnetic iron oxide and superparamagnetic lipiodol have good biosafety in vitro.Objective: To study the in vivo safety of different proportions of superparamagnetic lipiodol in hepatic artery embolization in New Zealand rabbits,and to explore the imaging of different proportions of superparamagnetic lipiodol in magnetic resonance imaging after operation.Materials and methods:12 New Zealand male rabbits with an average weight of3.3kg,were randomly divided into four groups: group A(ordinary lipiodol),group B(SPION: lipiodol = 1:2),group C(SPION: lipiodol = 1:1),group D(SPION: lipiodol= 2:1).After intubation through the right femoral artery,the microcatheter was placed in the left or right hepatic artery for hepatic artery embolization.Each experimental rabbit was injected with 0.6ml embolization material.MRI scanning was performed immediately after the operation,and the imaging differences of MRI scanning among the groups were compared.The liver and kidney functions(AST、ALT、UREA、CREA)of experimental rabbits were tested by blood sampling 1 day before operation and 7 days after operation,and the changes of liver and kidney functions before and after operation were compared.The pathological specimens of heart,liver,spleen,lung and kidney were observed after operation.Results: No significant abnormal signal changes were found in the liver MR scanning of experimental rabbits in each group.After measuring the T2 signal value through Leonardo post-processing,there was significant difference between group A and group D(t = 7.416,P = 0.000).The biochemical indexes of liver and kidney in each group were within the normal range 7 days after operation.The liver showed irregular coagulation necrosis after operation,and no obvious abnormalities were found in the rest.Conclusion: Different proportion of SPLIP has good biological safety in animals,and they have similar embolic effect with ordinary lipiodol.At the same time,T2 signal value of group D(SPION: lipiodol = 2:1)decreased during MRI scanning after operation.Combined with the previous research of our team,it is inferred that it can supplement the insufficient development of ordinary lipiodol in magnetic resonance imaging,which is conducive to guiding clinical practice. |
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