Research On The Synthesis Of Fluorescent Probe And Its Detection Of Metal Ions And Bovine Serum Albumin

In recent years,with the development of industrial processes,environmental pollution is becoming more and more serious,and environmental protection issues have been paid more and more attention.Hence,sensitive detection of environmental pollutants has become more and more important.The abnormal concentration of heavy metal ions and biological analytes caused by unreasonable discharge is a great threat to the natural environment and human health.Compared with other traditional detection methods,the fluorescence analysis method has the advantages of high selectivity,high sensitivity,good reproducibility,simple operation,nondestructive,and naked eye detection.A single probe responding to multiple analytes can save a lot of energy and material resources.Therefore,the development of fluorescent probes that can recognize and detect multiple analytes has more practical application value.To sum up,the following studies were carried out:1.The fluorescent probe DGly,which can detect Cu²⁺with high selectivity and sensitivity,was synthesized by one–step reaction,and its sensing performance was studied by fluorescence spectra,job’s plot,mass spectra,DFT calculation,fluorescence lifetime analysis,and other methods were used to explore the mechanism of DGly detection of Cu²⁺.Its ability to detect Cu²⁺in real samples was evaluated.2.A novel reactive fluorescent probe SHD with high selectivity and sensitivity for Cu²⁺detection was synthesized by one–step reaction using dansyl chloride and salicylic hydrazine.The response of SHD to Cu²⁺was studied by fluorescence spectra.In the presence of Cu²⁺,SHD hydrolyzed into dansulfonic acid.The emission peak intensity at 550 nm decreased with the increase of Cu²⁺concentration,and a new fluorescence emission peak appeared at 465 nm.The fluorescence color of the solution changed from yellow to blue under 365 nm UV light irradiation,which could achieve specific naked eye detection.3.Using polythiophene as the fluorophore and salicylyzide as the receptor,a novel multifunctional fluorescent probe SHTE with high selectivity and sensitivity for detection of Ni²⁺and recognition of Cu²⁺and Zn²⁺was synthesized by one–step reaction.Its sensing properties were studied by colorimetry and fluorescence analysis.The response of SHTE to a variety of metal ions was investigated by fluorescence and UV–vis spectra,and the response mechanism of SHTE to detect Ni²⁺and its ability to detect Ni²⁺in actual samples were explored.4.In chapter 2 and chapter 4,organic–metal ensemble DGly–Cu²⁺and SHTE–Ni²⁺were obtained,and the response of DGly–Cu²⁺and SHTE–Ni²⁺to biological analytes was studied by fluorescence spectra and UV–vis spectra.The results showed that the three–component conjugation of DGly–Cu²⁺with bovine serum albumin（BSA）could significantly increase the fluorescence intensity of DGly Cu²⁺at 540 nm.The response of DGly–Cu²⁺to BSA was studied by fluorescence analysis(F_{540 nm}).