Preparation And Properties Of Drug Sustained Release Carriers Based On Particle Of Mesoporous Silica

Objectives Nanodrug delivery system is a composite system with composed of nanoparticles and drugs.Among,mesoporous silica nanoparticles(MSN)are widely used in drug delivery systems due to their good stability and high drug loading performance.However,it is found that simple mesoporous silica is prone to drug leakage during the delivery process and lacks specificity.Therefore,the development of stimuli-responsive drug carrier based on MSN is particularly important.Methods 1 Photo-responsive drug carriers based on mesoporous silica: Firstly,mesoporous silica nanoparticles(MSN)were prepared by sol-gel method.Subsequently,(3-aminopropyl)triethoxysilane(APTES)and azobenzene-4,4’-dicarboxylic acid(AZO)were modified on the surface of MSN using grafting method.Finally,MSN@β-CD was self-assembled by inclusion interaction between AZO and β-CD.The best preparation process was investigated by single-factor method.Quercetin(QU)was used as a model drug to investigate the drug loading and drug releasing ability of the carrier.The biosafety of MSN@β-CD was investigated using hemolysis and CCK8 assays.2 p H/enzyme dual response drug carriers based on mesoporous silica: Firstly,the surface of MSN was modified with amination.Then,carboxymethyl chitosan(CMCS)and hyaluronic acid(HA)were sequentially modified by amidation reaction to prepare dual response drug carriers(MSN@HA).The optimal preparation process,drug loading,drug releasing and biosafety performance of MSN@HA were investigated.Results 1 The best drug loading performance was 16.7% when the mass of MSN-AZO,β-CD and QU dosage were 25.0 mg,23.0 mg and 10.0 mg,respectively,and the reaction temperature was 40°C.In vitro release study showed that the cumulative release rate of QU@MSN@β-CD after 72 h reached 26.09% under light,which was 2.14 times higher than the cumulative release under dark.2 The maximum drug loading was 23.93% when the mass of MSN@CMCS,mass ratio of EDC and NHS,amidation reaction time and QU dosage were 20.0 mg,2:1,18 h,and 5 mg,respectively.In vitro drug release results showed that the cumulative release rate of QU@MSN@HA at p H = 5 and hyaluronidase(100 μg/m L)was as high as 63.73%.3 In addition,the results of hemolysis assay showed that the hemolysis rate of both MSN@β-CD and MSN@HA was less than 5%.The CCK8 results showed that the cellular activity of both MSN@β-CD and MSN@HA was higher than 80%.Conclusions Two intelligent mesoporous silica-based nano pharmaceutical carriers(MSN@β-CD and MSN@HA)were successfully prepared by material structure design.And the drug release and CCK8 tests proved that the two carriers have good stimulated drug release and biosafety properties.Figure 45;Table 10;Reference 90...