Research On Key Technologies Of Pathogen Monitoring In The Air

ObjectiveThrough the development and optimization of the air particle sampler and the rapid detection platform of pathogenic organisms,and the evaluation of their effects,the key hardware support is provided for the construction of a fullautomatic monitoring and alarm system of pathogenic biological aerosol that integrates detection,collection and detection.Methods1.Development,optimization and performance evaluation of air particle sampler.On the basis of disassembling the components of the air particle sampler WA-15,the sampling module was developed and modified.On the basis of disassembling the components of the air particle sampler WA-15,the sampling module was developed and modified.Add a liquid replenishing pipe interface and a liquid discharging pipe interface on the collection pipe;Add two liquid guide pipes,which are respectively connected with the liquid supply interface and the liquid discharge interface;Two peristaltic pumps are added,and the two liquid guide pipes supplement and discharge liquid to the collection pipe through the peristaltic pump;Add two reagent bottles,one for supplementing the sampling solution to the collection tube,and one for collecting the sample solution discharged from the discharge tube;Various particle sizes(0.2μm、0.5μm 、 0.8μm 、 1μm 、 2μm 、 3μm 、 4μm 、 5μm)Polystyrene microsphere simulates the biological particles in the air aerosol with the same particle size.Polystyrene microsphere aerosol occurs in the test chamber.The particle counter is used to measure the concentration of aerosol with different particle sizes at the inlet and outlet of the sampling module,and the sampling efficiency(%)of the sampling module for the airborne biological particles with different particle sizes is calculated.2.Development,optimization and performance evaluation of rapid detection platform for pathogenic organisms.On the basis of disassembling the parts of UPT-3A,a rapid detection instrument for biological harmful factors,the detection card tray was developed and modified.A 3-channel tray is designed to hold 3 detection cards;A sliding table is designed to drive the detection card tray to move;The sliding table is combined with the up-turn luminous immunoassay module,and the continuous scanning detection of three detection cards can be realized by moving the sliding table.The slide is combined with the drain pipe of the sampling module to realize automatic filling of sample solution to the detection card;By preparing a series of standard bacterial solutions and negative blank control samples of different concentrations,a certain amount of samples were taken for up-transfer immunochromatography,and each concentration was tested three times in parallel,to evaluate the detection sensitivity and response time of the rapid detection platform for pathogenic organisms to bacteria and viruses.Results1.Development,optimization and performance evaluation of air particle sampler.The improved sampling module,combined with the air pump,power supply and control circuit,realizes the automatic replenishment and automatic drainage of the sampling device;And the automatic cleaning of the contaminated pipeline of the sampling device,which is conducive to the continuous sampling of biological aerosols;Air particle automatic sampling module half cutting particle size 0.5μm.The sampling flow is 14.1L/min.2.Development,optimization and performance evaluation of rapid detection platform for pathogenic organisms.The developed and modified 3-channel tray can hold 3 detection cards;The designed slide can drive the detection card tray to move;The slide is combined with UPT immune analysis module.Through moving the slide,three detection cards can be continuously scanned and detected.The slide is combined with the drain pipe of the sampling module,which can automatically fill the sample solution to the detection card;The response time of the pathogen rapid detection module to Yersinia pestis,Escherichia coli O157,Staphylococcus aureus and influenza virus is within 15 minutes(all 10min15s);The detection sensitivity for Yersinia pestis≥103 CFU/m L;Detection sensitivity of Escherichia coli O157 ≥ 105CFU/m L;Detection sensitivity of Staphylococcus aureus ≥ 106 CFU/m L;The detection sensitivity of influenza A virus≥0.25 HA.ConclusionThis research has successfully developed and optimized the air particle sampler and the rapid detection platform of pathogenic organisms,which provides preliminary preparation for the "automatic monitoring and alarm system of pathogenic biological aerosol" with integrated functions of real-time monitoring and early warning,full-automatic sampling and sample delivery,and identification and alarm,and also lays the foundation for effective monitoring of pathogenic biological pollution in the air of confined space.