Selective Separation And Enrichment Of CIS-dihydroxy Biomolecules By ZIF-based Solid Phase Extraction Adsorbent

Cis-dihydroxyl biomolecules are a class of compounds widely found in nature and living organisms,such as sugars,nucleic acids,nucleosides,glycoproteins,etc.Cis-dihydroxy biomolecules have various biological functions in living organisms,which can maintain the liquid balance and participate in cell metabolism.The study of cis-dihydroxyl biomolecules contributes to a deep understanding of the metabolic and regulatory mechanisms of organisms and is of great significance for biology,medicine,and other fields.However,the abundance of this class of molecules in real biological samples is very low and will be disturbed by the co-existing components with high abundance,so an appropriate method should be used to separate them.As a widely used separation and purification technology,the solid-phase extraction method has the advantages of fast adsorption and desorption speed and good compatibility.Therefore,this paper intends to establish an efficient solid-phase extraction technology for the separation and detection of cis-dihydroxyl groups in complex samples,to achieve the purpose of simple and rapid separation and analysis:1.The molecular imprinted polymer of ZIF fund was prepared（ZIF@PEI@Ti O2-MIP）,used for efficient and selective separation and enrichment of OVA in egg white samples,and investigated the effects of sample concentration,sample p H,ionic strength,and eluent type on the adsorption capacity.ZIF@PEI@Ti O2-MIP has rich imprinted sites,a large specific surface area,and a large adsorption capacity for rapid and accurate adsorption of OVA.The equilibrium adsorption time of OVA with the imprinted polymer was 35 min,and the maximum adsorption capacity of the material was 417.6 mg g-1.The imprinted site of the adsorbent can selectively identify the template OVA with good selectivity and adsorption specificity,and the adsorption process is consistent with the Langmuir adsorption model and the quasi-secondary adsorption model dynamics.The adsorbent with 0.5%CTAB as the eluent still has good adsorption performance after 6 adsorption elution cycles,which proves its good stability and reuse.The specific identification of imprinted materials of OVA in complex samples provides great prospects for the application of glycoproteins in both biological and clinical activities.A new type of organometallic skeleton boric acid modified molecularly printed polymer（ZIF@B@MIP）and glutathione-coated silver nanoparticles（GO@Ag@GSH）were prepared to extract natural polyphenols,EA,from discarded chestnut shell samples.First,EA is specifically captured by ZIF@B@MIP based on the borate affinity molecule.Meanwhile,silver on GO provides a large number of adsorption binding sites for EA.The synergy of the two materials has high sensitivity and adsorption capacity,better adsorption effect for EA than a single material,and better enrichment ability and selectivity for EA.The maximum adsorption capacity of the adsorbent was 46.25 mg g-1,The blot factor was 3.01 and the detection limit was0.020 mg L^-1,The spike recovery of this method is 95.6-110.1%.This study provides a new study protocol for the enrichment and purification of EA in discarded chestnut shells.