| Hydrogen sulfide(H2S)is an important neuroregulatory signaling molecule in the brain.It can not only directly regulate the central nervous system,but also indirectly affect the central nervous system through antioxidant,neuroendocrine and cerebrovascular functions.So far,many H2S fluorescent probes have been reported for in vitro,cellular and mouse abdominal imaging,but few H2S fluorescent probes involved in brain imaging.Therefore,it is of great significance to develop a brain-targeted H2S fluorescent probe for H2S imaging in brain-related diseases.Based on the above,the work of this paper is as follows:(1)Construction and performance of near-infrared H2S nanoprobes.H2S is susceptible to interference by endogenous biological thiols.Taking advantage of its unique dual nucleophilic properties,selenite sulfide(Se-S)as the recognition group to combine with near-infrared hemicyanine fluorophore to design a H2S probe which has high sensitivity and selectivity.In order to expand its ability to cross the blood-brain barrier,a brain-targeting near-infrared nanoprobe BFPP was constructed by encapsulating the small molecule probe based on polymer self-assembly strategy,and the optical performance of the nanoprobe to H2S was explored.BFPP has excellent fluorescence properties,which can realize H2S-specific imaging,and has potential bioimaging application prospects.(2)Bioimaging applications of near-infrared H2S nanoprobes in epilepsy diseases.Endogenous H2S plays an important role in the pathogenesis of nervous system diseases.H2S in the normal concentration range has both important regulatory and neuroprotective effects on the central nervous system,and too high or too low concentrations of H2S can affect the normal function of the nervous system.This work established a epilepsy mouse model induced by pentylenetetrazole(PTZ),the correlations between H2S levels and severe neuronal damage and epileptogenesis were investigated by BFPP,and the fluorescence imaging of endogenous H2S was performed in the brains of epileptic mice. |
