Construction Of Conductive Microenvironment Based On Carbon-based Materials And Its Role In Myocardial Repair

BackgroundThe incidence rate of cardiovascular disease(CVD)is currently one of the leading causes of global morbidity.Ischemic heart disease is the most common form of cardiovascular disease,mainly manifested as a decrease in coronary blood flow and eventually leads to myocardial infarction(MI),which affects More than seven million people worldwide each year.In recent years,biomaterial based treatment methods have attracted more and more attention in the treatment of myocardial infarction.Many biological functional materials are used to make myocardial patches,and have been proved to promote cardiac recovery through mechanisms such as structural strengthening,neovascularization or reduction of inflammation.However,they usually lack an important component,namely conductivity,which is a very important factor in the process of myocardial repair.Therefore,in order to further improve the mechanical properties of various biological scaffolds,the introduction of carbon based materials will play a very significant role and have great application potential in tissue engineering.At present,the use of carbon based materials to construct conductive microenvironment has also been confirmed by many studies that it can greatly improve the repair ability of myocardium,which has good medical significance and research prospects.ObjectiveA variety of conductive microenvironments based on carbon based materials were constructed by tissue engineering to promote myocardial infarction repair.Methods1.Programmable PVA/CNT/Elastin elastic conductive fibers were prepared by wet spinning.The characterization,properties and effect on cells of the prepared elastic conductive fiber materials were studied.2.To study the effect of graphene conductive myocardial patch obtained by 3D printing on cells.3.A rat model of myocardial infarction was established,and 3D printed graphene conductive myocardial patch was implanted in the infarct area to study its repair effect on myocardial infarction in rats.Results1.The diameter of the prepared programmable PVA/CNT/Elastin elastic conductive fiber is 200-500μm,which is in a regular strip shape.It has good weaving performance,conductivity and biocompatibility,and is suitable for the growth of cardiomyocytes.2.3D printed graphene myocardial patch provides a good conductive microenvironment for cardiomyocytes,has high biocompatibility,and can greatly promote the functionalization of cardiomyocytes,improve the expression of related proteins,and promote angiogenesis in vivo and in vitro.3.After 3D printed graphene myocardial patch was transplanted into myocardial infarction rats for 4 weeks,the cardiac function of rats was significantly improved.Histological methods proved that the myocardial patch can promote the angiogenesis of myocardial infarction area,reduce the area of myocardial infarction,improve the expression level of myocardial maturation related proteins,and have significant myocardial repair function.ConclusionThe myocardial patch prepared based on carbon based materials can form a good conductive microenvironment,which is suitable for the growth of cardiomyocytes and has a significant effect on myocardial infarction repair.It is confirmed that carbon based materials have a good development prospect and application potential in myocardial tissue engineering.