Preparation And Antioxidant Activity Of Eel Nanoparticle-anthocyanin Complex

Eel has extremely high nutritional value and medicinal value.In recent years,researchers have paid more attention to the nutritional value of eels,and only a little research has been done on the application of natural nanoparticles in delivery systems.Food-derived nanoparticles are similar to CDs with unique physicochemical properties and higher biosafety and biocompatibility.They are excellent carriers for protecting active substances as a delivery system.It has broad application prospects in the field of food functional factor delivery.Anthocyanins have good physiological functions,such as antioxidant activity,antibacterial activity,lowering blood pressure,etc.Most anthocyanins in nature exist as unstable sugar ligands,which are easily degraded by the environment.Particles adsorb or encapsulate anthocyanin particles to protect the stability of anthocyanins and reduce their oxidation and decomposition rates.In recent years,the use of nanoparticles to improve the stability and functionality of anthocyanins has become a research hotspot.Therefore,it is an urgent problem to study the homeostasis of anthocyanins and improve their bioavailability through the complexation of eel nanoparticles（ENPs）with anthocyanins.In this study,eel and cornflower-3-glucose（CYD-3-G）were used as raw materials to prepare eel nanoparticles-anthocyanin complexes（ENPs-C3G）.The structure of ENPs was characterized by scanning electron microscopy and dynamic light scattering.The ENPs-C3G complexes were identified by scattering and UV-Vis spectroscopy,the biostability of anthocyanins was evaluated by stability experiments and in vitro digestion experiments,and the antioxidant activity of ENPs-C3G complexes was investigated by in vitro antioxidant experiments and Hep G2 cell oxidative damage protection.The specific research contents and main results are as follows:1.The ENPs extracted by ethanol extraction were compounded with CYD-3-G,and the scanning electron microscope results showed that ENPs were spheroid-like particles.The results of encapsulation efficiency measurement showed that the encapsulation efficiency of ENPs to CYD-3-G was 37.47±1.53%.The turbidity measurement results showed that the turbidity of the ENPs-C3G complex was significantly higher than that of CYD-3-G,and macromolecular substances were produced in the complex system.The results of UV-Vis spectral scanning analysis showed that the maximum absorption peak of ENPs-C3G complexes had an obvious blue-shift phenomenon compared with CYD-3-G,which confirmed the formation of complexes between ENPs and CYD-3-G.The results of dynamic light scattering（DLS）analysis showed that the particle size of ENPs-C3G complexes was 683.30±2.031 nm,the zeta potential was-25.43±0.170 m V,and the polydispersity coefficient（PDI）was0.464±0.004,which Compared with CYD-3-G,the particle size is significantly larger,theζ-potential is increased by about-35 m V,the polydispersity coefficient（PDI）is smaller,and the system is relatively stable.2.The ENPs-C3G complexes in the ENPs-C3G complexes under the conditions of light（LED light）,normal temperature（25°C）and different p H values（3.0,4.0,5.0,6.0,7.0）,with the prolongation of storage time,the Shows a protective effect on the stability of CYD-3-G.3.The simulated in vitro digestion results showed that during the simulated oral digestion,the anthocyanins in the ENPs-C3G complex and CYD-3-G samples were not significantly degraded,and the retention rate and FRAP reducing power measurement results did not clearly reflect the ENPs-C3G Protection of CYD-3-G by the complex.During simulated gastrointestinal digestion,anthocyanins were significantly degraded in both groups of ENPs-C3G complexes and CYD-3-G samples.The results of retention rate and FRAP reducing force assay showed that ENPs-C3G complexes in gastric juice and intestinal juice had a negative effect on anthocyanins.CYD-3-G has obvious protective effect.4.In vitro antioxidant activity of ENPs-C3G complexes:The results of ABTS+·scavenging ability assay showed that the ABTS+·scavenging ability of ENPs-C3G complexes was significantly higher than that of CYD-3-G,and the ABTS+·scavenging ability of 1:20 ENPs-C3G complex was the strongest,which was close to 100%.The FRAP reducing power measurement results show that the reducing power of ENPs-C3G to ferrous ions is higher than that of CYD-3-G,especially the reducing power of ENPs-C3G ferrous ions formed by CYD-3-G:ENPs in a ratio of 1:40.0.40±0.033μmol/g,which was significantly higher than that of CYD-3-G,which was 0.34±0.03μmol/g for the reduction of ferrous ions.5.ENPs-C3G complexes protect Hep G2 cells from oxidative damage:ENPs-C3G has no cytotoxicity when the final concentration is25-400μg/m L and 400,200 and 100μg/m L were selected for subsequent experiments.The concentration of H₂O₂was 200μM,and the cell viability was 60.5%when treated for 6h,which met the modeling conditions.Compared with the model group,ENPs-C3G complexes showed significant decrease in ROS level and NO content in Hep G2 cells induced by H₂O₂.ENPs-C3G complex has a certain protective effect on H₂O₂-induced oxidative damage in Hep G2 cells.