Biological Effects Of Nano-agrochemicals

The contemporary world,people are facing the problems of population growth,food demand and environmental pollution,nano-agrochemicals are considered as the viable and alternative to solve this problem.Therefore,this study prepared a kind of nano-agrochemical CV@n Ti O₂ based on inorganic nanoparticles Ti O₂ NPs as the carrier and crystal violet CV as the active component,which had p H-and temperature-responsive release characteristics.Both foliar and soil application of CV@n Ti O₂ on tomato seedling showed that its promotive effect on tomato seedling growth was better than that of traditional agrochemical CV,and soil application could increase the microbial diversity and microbial species abundances in the rhizosphere soil.In addition,CV@n Ti O₂ could be translocate from the initially exposed zone to other plant compartments with foliar application,or translocate from roots to shoots with soil application,where Ti concentration in roots of tomato seedlings was more than 10 times higher than that in shoots.We found the transmission efficiency of Ti element in plants was decreased with the increase of CV@n Ti O₂ concentration with soil application.Meanwhile,the biological effects of CV@n Ti O₂ on Chlorella were studied.The results showed that the inhibition rates of CV@n Ti O₂ on cell growth and chlorophyl-a synthesis of Chlorella were less than 5%in the range of 0.1-10 mg L^-1,and was lower than CV used as conventional agricultural chemicals at the same concentration level.The analysis of extracellular secretion and intracellular macromolecule substances of Chlorella showed that they increased with the increase of CV@n Ti O₂ concentration.Comparing the biological effects of CV,Ti O₂ NPs,CV@n Ti O₂ and CV+Ti O₂ NPs at same concentration on Chlorella,it was found that the inhibition rate of chlorophyll-a synthesis was negatively correlated with the content of extracellular secretion of Chlorella.The results of this study provide new ideas to solve the efficiency and environmental loss of traditional agricultural chemicals in the use process,provide basic data for the biological effects of nano-agrochemical,and provide research basis for ecological risk assessment of nano-agrochemicals in practical applications.