Improvement Effect Of Agrocybe Aegirita Polysaccharides And Flavonoids On DSS-induced Colitis

Inflammatory bowel disease(IBD)is an idiopathic intestinal inflammatory disease that mainly affects the normal metabolism of the body in the ileum,colon,and rectum.Agrocybe aegirita(Agrocybe aegirita),also known as columnar field mushroom,is a kind of fungus belonging to Basidiomycetes Agaricaceae,mainly distributed in subtropical and north temperate regions.It is rich in Jiangxi Guangchang and Fujian Gutian in my country.High nutritional value and a variety of biological activities,including anti-cancer,antioxidant,anti-inflammatory,immune regulation and other effects.At present,it has been confirmed that polysaccharides and flavonoids are the main functional factors of fungal ingredients,and have the functions of enhancing immunity,anti-inflammatory,and anti-oxidation,but there are few reports on the functional factors of Agrocybe aegirita.Therefore,in this study,the Agrocybe aegirita was used as the raw material to prepare and obtain Agrocybe aegirita waterextracted polysaccharide(SAAP),Agrocybe aegirita alkali-extracted polysaccharide(JAAP)and Agrocybe aegirita by water extraction and alcohol precipitation method,alkali extraction and alcohol precipitation method and organic solvent extraction method.Flavonoids(English abbreviation),to determine the basic physical and chemical properties of three functional factors.Afterwards,based on the mouse colitis model,the improvement effects of three functional factors on colon injury and inflammatory response in mice with colitis were evaluated and compared,and the possible differences in the mechanisms of action of the three functional factors were analyzed from the perspective of intestinal flora.This study provides a certain theoretical support and scientific basis for the in-depth development and utilization of Agrocybe aegirita resources.The main research contents and results of this paper are as follows:(1)The Agrocybe aegirita polysaccharide was extracted by the methods of water extraction and alcohol precipitation and alkali extraction and alcohol precipitation to obtain the Agrocybe aegirita water-extracted polysaccharide SAAP and the Agrocybe aegirita alkali-extracted polysaccharide JAAP.The neutral sugar content of the two polysaccharides was determined by the phenol-sulfuric acid method,the acid sugar content was determined by the carbazole-ethanol method,the protein content was determined by the Coomassie brilliant blue method,and finally the high performance anion exchange chromatography tandem pulsed amperometric detector(HPAEC-PAD)was used analysis of monosaccharide composition.(2)The crude flavonoids of Agrocybe aegirita were obtained by ethanol extraction,and then purified by static adsorption with macroporous resin.From 13 macroporous resins,HPD-400 was screened to have the best static adsorption effect.Then,the macroporous resin was used to purify and enrich the flavonoids.to 0.0534 mg/ml,The flavonoid content in the freeze-dried sample was 21.18%.(3)Based on DSS-induced colitis in mice,the effects of three functional factors of Agrocybe aegirita on intestinal injury and inflammatory response in model mice were compared.The results showed that: First,SAAP and JAAP could not significantly delay the weight loss trend and reduce the DAI index in mice.and spleen index,AAF can delay the trend of weight loss,reduce DAI index and spleen index in mice;secondly,SAAP and JAAP have no significant effect on improving colon length shortening,AAF can have a significant effect on colon shortening;in addition,SAAP and JAAP have a significant effect on colon shortening Can significantly increase the content of IL-10,JAAP can significantly reduce the content of IL-1β,AAF can increase the content of IL-10,reduce the content of IL-1β and reduce the content of TNF-α;finally,SAAP and JAAP can significantly reduce the content of colon pathology.There was no significant effect on tissue and intestinal barrier function.AAF could improve colon pathological tissue and intestinal functional barrier in colitis mice by increasing mucin secretion and the number of goblet cells.(4)Based on 16 S high-throughput sequencing technology,the effects of SAAP,JAAP and AAF on the intestinal flora of colitis mice were analyzed;the results showed that at the phylum and genus levels,AAF may regulate Lactobacillus and Ackermann by regulating Compared with the model group and the control group,the tea tree mushroom polysaccharide also significantly increased in the regulation of Akkermansia.But compared with flavonoids,its ability to regulate Akkermansia and Lactobacillus is weaker.