Study On The Mechanism Of Treating Two Kinds Of Micro-pollutants With Fe₃C Enhanced Electroactive Constructed Wetland

This study took sewage treatment and resource as the research direction,constructing electroactive constructed wetland（ECW）,that is,constructed wetland microbial fuel cell（CW-MFC）as research object.The experiments were carried out by using Fe₃C to optimize device water inlet and the strengthen of the anode material enriched anode electronic donor.The wastewater purification was evaluated by analyzing the removal efficiencies of pollutants in the water and the target pollutants.The power generation function was evaluated by monitoring the external voltage of the device during the entire experimental process.At the end of the experiment,conduct the polarization curve test to obtain device internal resistance and the power density.The device inoculation sludge samples and the microbial samples were taken after the two sets of devices at stable period for 16s r RNA high-throughput sequencing.The microbial community diversity and the functional potential prediction analysis were carried out based on the existing data.The water purification effect and power generation efficiency of the device were further evaluated by combining the sample extracellular polymers.Fe₃C enhances water purification and electricity production by improving functional microbial community.There is no significant difference in the diversity of the anode and cathode microbial community,but the microbial community diversity of the device anode was higher than the device cathode.The microorganisms have also changed.Due to the addition of iron,the anaerobic ammonia oxide bacteria（Pseudomonas,Geobactor）in the device were also significantly improved.The percentage of electroactive bacteria represented by Proteobacteria and Geobactor increased in the community.The percentage of functional bacteria related to nitrogen and phosphorus conversion was also improved.Based on the high-throughput analysis results,the microbial functional potential was predicted.The results showed that the proportion of enzymes related to energy conversion in the anode microorganisms of the device increased significantly.The content of cofactors and vitamins increased in iron-related treatment devices.Cofactors and vitamins contain riboflavin which is an important electronic mediator.It can accelerate electron transport,thereby improving the power production performance of the device.This study provides theoretical support for Fe₃C pretreatment of iron-containing wastewater in large-scale constructed wetlands water purification and electricity production.Fe₃C can improve the treatment of BPA wastewater by enriching the anode electron donor,improving the metabolic activity of microorganisms,optimizing the electrochemical process of microorganisms.This technology has potential practical application value.