Porphyrin-based Metal-organic Nanoparticles Synthesized In An Aqueous Solution And Their Antitumor Studies

Porphyrin-based metal-organic nanoparticles have great application prospects in cancer diagnosis and treatment due to their porous loading,excellent photosensitivity,and multi-component synergistic effects.However,the commonly used synthesis methods of porphyrin-based nanoparticles are mostly solvothermal methods,and the solvents are mostly organic solvents such as methanol and are accompanied by high temperature and high-pressure conditions.These conditions bring potential toxicity to the synthesized nanoparticles or lead to the inactivation of the loaded guest active drug molecules under heating conditions,making it difficult to meet the requirements of antitumor diagnosis and treatment.To solve the above shortcomings,it is very necessary to expand the application of porphyrin derivative-based metal-organic nanoparticles in anti-tumor and to explore the preparation method of porphyrin-derivative-based metalorganic nanoparticles in aqueous solution for tumor diagnosis and treatment.In this paper,metal-porphyrin-based nanoparticles with good biocompatibility were prepared under mild aqueous solution conditions by two strategies:synthesis of water-soluble porphyrin ligands and inverse microemulsion method,avoiding high temperature and high-pressure synthesis conditions and the problem of using a large amount of organic toxic solvents,and the anti-tumor synergistic therapy of metalorganic nanoparticles has been realized.Based on this,the following work has been mainly carried out:1.The first part aims to design and construct water-soluble porphyrin ligand molecules,try a variety of polar coordinating groups,and covalently combine with the porphyrin parent to synthesize a porphyrin polydentate ligand PDZ with good water solubility,In the aqueous system,it can coordinate with transition metals to form metalorganic nanoparticles,and achieve high-efficiency photodynamic therapy.It also provides a good drug loading platform for loading guest drug active molecules.2.In the second part,a general and simple inverse microemulsion method is used to synthesize nanoparticles with good biocompatibility.The water-soluble bimetallic organic nanoparticles FeMn-TCPP NPs can be mildly and effectively prepared by the inverse microemulsion method.The nanoparticles showed good stability in biological buffers and culture fluids.Simultaneously,synthesized nanoparticles integrate magnetic resonance imaging,photodynamic therapy,and chemodynamic therapy into the same platform,showing excellent antitumor properties and application potential in tumor therapy.