Establishment Of Cell And Animal Detection Models For Curcumin Antioxidativity

The oxygen free radicals(ROS)are the main free radicals in the body.The accumulated free radicals will attack the biomacromlecules,leading to oxidative stess when their production and scavenging system were out of balance.Curcumin is a plant-derived polyphenol with strong antioxidant activities,however the underlying mechanism remains to be elucidated.Lack of appropriate research models has hindered its biological functions exploration.In this study,low-differentiated,high-differentiated pheochromocytoma cells(PC 12)and aged mice were used for oxidative stress model establishment to address the antioxidant mechanisms.The study may provide insights into the application of curcumin and other functional components.The main research results are as follows:(1)An oxidative stress cell model was established in H2O2-stimulated PC 12 cells to investigate the antioxidant activity of curcumin.Upon 24 h curcumin treatment,the total antioxidant capacity(T-AOC),the activities and expression of major antioxidant enzymes(SOD,CAT,GSH-Px)were increased.In addition,curcumin pretreatment significantly reduced intracellular ROS level and increased T-AOC as well as the activities and expression of major antioxidant enzymes in H2O2-stimulated cells.These results indicated that the intervention of curcumin effectively reduced H2O2-stimulated cells oxidative stress.(2)The mouse model was established in aged mice for detection of curcumin antioxidation ability.The results showed that curcumin effectively increased plasma T-AOC and major antioxidant enzyme activities in a dose-dependent manner.Moreover,the major antioxidant enzyme activities were significantly increased in mouse livers.The mitochondrial transcription factors(PGC-1α,ERRα,TFAM),the mitochondrial-coding genes(ND1,ND5,COX1,COX3)and the antioxidant enzymes(GPX1,CAT,SOD2)were also up-regulated,indicating that curcumin alleviates oxidative stress by regulating the antioxidant enzymes and mitochondrial biosynthesis.In summary,curcumin could remove excess ROS from the body,and up-regulate the activities and expression of major antioxidant enzymes,which leads to enhanced total antioxidant capacity.The mitochondrial homeostasis may be maintained by curcumin via its biogenesis regulation,which promotes the redox system stability and eventually alleviates the body’s oxidative stress.