| Natural enzymes have excellent catalytic performance under mild reaction conditions.However,the practical application of natural enzymes is usually hampered by their drawbacks,including high costs in preparation and low stability.Artificial enzymes can not only mimic the catalytic function of natural enzymes,but also eliminate the limitations of the applications of enzymes.As a peroxidase mimic,hemin itself is hydrophobic and prone to forming aggregates in aqueous solutions,which limits its direct applications in aqueous systems.To solve these problems,construction of water-soluble hemin-based artificial enzyme is a commendable way.In this thesis,we first prepared a highly water-soluble hemin covalently functionalized carbon-based artificial enzyme(CN-Hemin)by a one-pot method,and explored the peroxidase-like activity,photocatalytic activity and its application.The main work can be summarized as follows:(1)Hemin covalently grafted carbon-based nanomaterial was prepared via one-pot pyrolysis of the mixture of citric acid and hemin.The as-prepared hybrid could overcome the shortcoming of hemin,such as its poor water solubility and easy agglomeration.Additionally,our strategy was also reasonably straightforward without the use of any toxic reagents.(2)The obtained artificial enzyme(CN-Hemin)possessed good water-solubility,intrinsic peroxidase-like activity and satisfactory photocatalytic performance in visible light.These extraordinary properties were mainly due to the structure of CN-Hemin containing iron porphyrin,aromatic carbon,and abundant oxygen-containing functional groups.(3)Under visible light irradiation,the prepared hemin-incorporated nanohybrid could generate abundant reactive oxygen species via the combination of peroxidaselike activity and photocatalytic performance,and thus can be applied to synergistically degrade dye and kill Staphylococcus aureus as well as Methicillin-resistant Staphylococcus aureus.Both dye removal rate and sterilization rate were over 90%. |
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