| In this experiment,nitrogen fertilizer were respectively applied on vinifera wine grapes Cabernet Sauvignon with 0、10、20、30、40g/plant treatment.Our objective was to study the effects of different nitrogen level on nitrogen compounds content and nitrogen metabolism key enzymes activity of the grape leaf.The results showed that:1.Compared to CK,during grape ripening,total nitrate nitrogen,ammoniacal nitrogen,soluble protein content and nitrate reductase(NR),glutamine synthase(GS)activity of Cabernet Sauvignon grape leaf were significantly increased with four kinds of nitrogen treatments.Followed by 40 g/plant>30 g/plant>20 g/plant>10 g/strains,And 40 g/plant nitrogen fertilizer treatment significantly higher than those of CK and other treatments.2.Compared to CK,during grape ripening,ammonium nitrogen,total nitrate nitrogen,ammoniacal nitrogen,soluble protein content and nitrate reductase(NR),glutamine synthase(GS)activity of Cabernet Sauvignon grape berry were significantly increased with four kinds of nitrogen treatments.Followed by 40 g/plant>30 g/plant>20 g/plant>10 g/strains,And 40 g/plant nitrogen fertilizer treatment significantly higher than those of CK and other treatments.3.Compared to CK,during grape ripening,reducing sugar,totle acid,single fruit-weight,totle tannin,totle phenolics and totle anthocyanin content of Cabernet Sauvignon grape berry were significantly increased with four kinds of nitrogen treatments.Followed by 40 g/plant>30 g/plant>20 g/plant>10 g/strains,And 40 g/plant nitrogen fertilizer treatment significantly higher than those of CK and other treatments.4.Compared to CK,during alcoholic fermentation,specific gravity,reducing sugar,assimilation nitrogen,saccharomycetes of must were significantly increased with four kinds of nitrogen treatments.Acoholicity and extract of wine were also increased.Followed by 40 g/plant>30 g/plant>20 g/plant>10 g/strains,And 40 g/plant nitrogen fertilizer treatment significantly higher than those of CK and other treatments.In a word,with nitrogen fertilizer were increased,the quality of the wine was improved. |