| Objective This study intends to evaluate the effects of different doses of beef decoction(BD)on rats with cerebral ischemia from the following two aspects such as brain injury and cellular immune function,and to explore the relation between brain injury and cellular immune function in order to investigate the mechanism of BD which protects cerebral ischemia from cellular immune injury,provide the evidence for clinical treatment of cerebral ischemia and theoretical guidance of diet for clinical cerebral ischemia patients.Methods 106 male SD rats(SPF scale)were randomly divided into six groups:the first sham-operated group,the second sham-operated group,model group,low dosage BD-treated group[200 mg/(kg·d)],middle dosage BD-treated group[400 mg/(kg·d)]and high dosage BD-treated group[600 mg/(kg·d)].Rats were received the same volume of normal saline(NS)in the first shame-operated group and model group,and the other rats were received the high dosage of BD in second shame-operated group.The middle cerebral artery occlusion(MCAO)was induced in the model group and the three BD-treated groups using the suture method.The body weight of rats was observed before surgery and at 7days after surgery.The neurological deficit score was used to evaluate the neurological function,TCC staining was used to label infarct volume,and HE staining was used to observe morphological changes of brain at 7days after surgery.The peripheral blood T lymphocyte subsets and NK cells were detected by flow cytometry at 7days after surgery.Result1 Compared with the sham group,the body weight of rats was significantly decreased at 7 days after surgery(P<0.01)and statistically increased after high dosage BD treatment(P<0.05).Compared with the model group,the mortality rate among the BD-treated groups were not significantly different(P>0.05).2 The infarct volumes of the middle and high dosage groups were significantly smaller than the model group(P<0.01).Compared with the model group,the neurological deficit scores of the BD-treated groups were not significantly different at baseline and 7 days after surgery(P>0.05).The changes of ischemic impairment in BD-treated group were lighter than that of model group.3 Compared with the sham group,the weight of spleen and spleen index were significantly decreased at 7 days after surgery(all P<0.01)and statistically increased after high dosage BD treatment(all P<0.01).The levels of CD3+,CD4+,CD4+/CD8+and NK in the model group were significantly lower than the first sham-operated group(all P<0.01),while the levels of CD3+,CD4+,CD4+/CD8+and NK in the high dosage group were lower than those in the model group(P<0.05、P<0.01、P<0.01 和 P<0.05).4 The infarct volume had negative correlation with the levels of CD3+,CD4+and CD4+/CD8+(r=-0.439,P=0.041;r=-0.453,P=0.034;r=-0.741,P=0.000)and positive correlation with the neurological deficit score(r=0.556,P=0.06).The infarct volume had no correlation with the level of CD3+(r=0.216,P=0.549).The infarct volume had no correlation with the level of CD8+and NK cells(r=0.326,P=0.139;r=-0.393,P=0.070).The neurological deficit score had negative correlation with the levels of CD3+,CD4+ and CD4+/CD8+(r=-0.506,P=0.016;r=-0.522,P=0.013;r=-0.448,P=0.014)and no correlation with the level of CD8+and NK cells(r=0.149,P=0.508;r=-0.261,P=0.232).Conclusion1 BD can reduce weight losing and infarct volume of rats with cerebral ischemia,improve scores of neurological deficits scales and reduce tissue damage after cerebral ischemia.2 High dosage of BD can significantly improve the level of CD3+,CD4+,CD4+/CD8+and NK cells in peripheral blood,thus regulating peripheral blood cellular immune function after cerebral ischemia of rats.3 The infarct volume and the neurological deficit score have negative correlation with the levels of CD3+,CD4+and CD4+/CD8+.The infarct volume also has positive correlation with the neurological deficit score.The more severe brain damage,the more severe immune dysfunction in peripheral blood cells of the body. |
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