| Foxa2 is a transcription factor containing the conserved sequence and DNA binding domain.Foxa2 plays an important role in early animal development,regulating the differentiation and development of a variety of cells and tissues,and contributes to the occurrence,malignant transformation and progression of tumors,as well.Although current studies have revealed the functions of Foxa2 on development from various aspects,but it is still not clear how Foxa2 acting as a pioneer transcription factor affects different developmental processes.Further study is urgently needed to extend our understanding of the Foxa2 functions on embryonic development and malignant transformation.This study has established foxa2 knockout zebrafish lines by CRISPR/Cas9.Based on the foxa2 mutant zebrafish and transcriptome analysis data,I investigated the function of foxa2 on embryo development using phenotypic analysis,gene in situ hybridization and quantitative PCR.The main results were listed as follows:（1）Two foxa2 mutant lines containing different frameshift in open reading frame were obtained.（2）foxa2-/-（F2 generation）showed severe phenotype with curly tail,abnormal development of nervous system,digestive system and blood vessel.（3）Knockout of foxa2 disturbed the expression of m RNA processing regulatory genes including nova2,rbfoxl1,pabpc4,and rbm4.3 in 24-27 hpf zebrafish embryos,which may be related to the abnormal development of somite and nervous system.In conclusion,this study has established the stable foxa2 mutant line of zebrafish.The functional loss of foxa2 resulted in severe malformation of zebrafish embryos and disturbed the expression of m RNA processing genes.An animal model has been established for studying the mechanism of foxa2 during the early development of embryos. |
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