Study On The Influence Of Strong Vibration Coupling On DNA Self-assembly

Over the past decade,scientists have found that when an optical transition is excited by a confined optical mode oscillates at the same frequency,a strong coupling between the two vibrations can occur,resulting in a polarized state of light and matter.The process is related to the zero-point fluctuation of the optical mode and the molecular transition.This phenomenon,known as vibrational strong coupling(VSC),has been exploited by scientists to manipulate chemistry and materials with significant progress.Biological responses are an integral part of the movement of life.In theory,by placing a biological reaction in an optical cavity,the optical mode of the cavity can be coupled to the energy of the biomolecules to control the biological reaction,even in the dark.However,these experiments are not easy to perform correctly,so studies on the biological response of VSC are very scarce and rarely reported.In this paper,we focus on the effect of strong vibrational coupling of pyridine groups in DNA on DNA self-assembly.The choice to use DNA origami as a template is a DNA self-assembly technique in which a long strand of DNA containing thousands of bases is folded into a target shape by complementary base pairing through hundreds of short single-stranded DNA.The sequence-dependent,accurate hybrid origami conformation can be observed by atomic force microscopy(AFM)and accurately measured by image processing.Some studies have shown that terahertz light can promote the breaking of DNA hydrogen bonds and reduce the melting temperature.However,VSC involves coupling molecular vibrations with electromagnetic radiation in the mid-infrared or terahertz region.Therefore,we first studied how to construct the FP cavity to meet the experimental requirements in the laboratory,and built a microfluidic control system to control the flow of sample solution in the FP cavity.We then performed strand displacement experiments using DNA short strands carrying fluorophores and quenched groups to explore the strong coupling conditions.Finally,we performed DNA self-assembly experiments in the FP chamber.The experimental results show that the optical mode of the FP cavity is strongly coupled to the pyridine group,which effectively promotes the hydrogen bond breaking and significantly reduces the temperature required for DNA origami.The 8.5 μm spacing FP cavity is equivalent to being in the mid-infrared(MIR)light range.It has the optimal enhancement effect for directly driving DNA origami co-assembly in the FP cavity.This work contributes to the study of quantum mechanisms in life and provides new implications for the development of innovative Mir-based techniques to regulate life applications.It is hoped that in the future,VSC can be applied to a wider range of biological reaction studies to improve the understanding of the biochemistry driven by VSC.On this basis,a new VSC technology applied in medical treatment,imaging and other aspects is developed.We believe that VSC has a broad prospect in the near future.