Research On Terahertz Optical System For Cell Stimulatio

Because terahertz waves have similar resonance frequencies to biomolecules,have low energy characteristics,and do not cause ionizing damage,terahertz technology has unique advantages in the biomedical field.Compared with the progress of terahertz waves in the field of medical diagnosis and imaging,the application of terahertz technology in the field of treatment and rehabilitation is still in the exploratory stage.The use of terahertz radiation technology to develop treatment and rehabilitation methods,especially for neurological diseases,will provide a new theoretical basis for the diagnosis and treatment of diseases.In practice,because skin,muscle and biological tissues will affect the energy transmission of terahertz waves,and the existing terahertz irradiation system is mainly for the improvement of terahertz sources,there is less research on the lens group of the optical system itself.Therefore,relying on the actual scientific research projects of the research group,this paper carried out the relevant research on terahertz optical system for cell stimulation,the main contents are as follows:(1)Considering the energy loss of terahertz waves in the transmission process,this paper constructs a terahertz optical system in the optical simulation software TracePro according to the characteristics of terahertz waves,and compares the advantages and disadvantages of the three light sources and combines the characteristics of laboratory terahertz lasers to find that the lattice point light source has a high fit with the laser used in this laboratory,which is conducive to matching the simulation with the actual light source,so the lattice point light source is selected as the simulation light source.At the same time,high-resistivity floating silicon coated with paraxylene antireflection coating on the surface was selected as the terahertz lens material,and through software design and multiple ray tracing experiments,the lens parameters were continuously optimized,and a terahertz optical system composed of three lenses was established.(2)In order to adapt to the needs of different research subjects and enable terahertz waves to be accurately irradiated to the nerve nuclei,it is necessary to design a terahertz optical system that can achieve effective focusing in a long focal length and wide range.The terahertz optical system designed in this paper can perform terahertz irradiation experiments for different biological samples,and can focus well in the range of330mm～420mm from the third lens to the image surface,and can concentrate energy to multiple target positions more consistently point by point in the range of 90 mm.At the same time,the system can also control the degree of defocus of the system by adjusting the position of the second lens,thereby changing the energy intensity of the terahertz wave at the sample plane and the size of the spot.(3)In order to verify the strong penetration of terahertz,the experimental results show that the terahertz wave can pass through the wooden box,knife and human hand,etc.,which proves the strong penetration of terahertz wave and meets the research needs.At the same time,according to the simulation results,a terahertz lens was customized,a terahertz optical system for cell stimulation was built,and biological cell irradiation experiments were carried out using this system.Rat adrenal pheochromocytoma cells(PC-12)cells were selected as experimental objects,and the experimental results showed that a certain intensity of terahertz waves promoted the activity of PC-12 cells and partially antagonized the decrease in PC-12 cell activity caused by Rotenone.In summary,this paper designs a terahertz optical system for cell stimulation,which studies the transport characteristics and energy analysis of terahertz in complex biological structures,and conducts simulation analysis and experimental verification,which preliminarily proves that the terahertz optical system can meet the requirements of terahertz energy radiation research and can be widely used in various terahertz radiation experiments in biological tissues or cells.The research conclusion provides a new idea for the potential application of terahertz in the field of biomedicine,and provides a basis for the development of non-invasive diagnosis and treatment of neurological diseases based on terahertz means.