Study On The Structure-activity Relationship Of Fern Hemp Polysaccharide Molecules

With the deepening of biological research,the structure-activity relationship of biological macromolecules has become a difficult problem and core problem in the field of biology,with the rapid development of computer technology,with the help of computer technology can greatly help biologists to solve biological problems,such as the spatial structure and structure-activity relationship of protein biological macromolecules,which makes the study of structure-activity relationship of biological macromolecules rise to a more systematic level.At present,the structure-activity relationship between protein macromolecules and nucleic acid macromolecules has been studied comprehensively and extensively.Now more and more studies have found that plant natural polysaccharides extracted from plants have significant effects in anti-radiation,anti-hypoxia,antioxidant and anti-virus,so that more and more biologists have begun to focus on the study of plant natural polysaccharides,wanting to explore the role played by plant natural polysaccharides in the life process.Nettle is a special plant in the plateau area,rich in polysaccharides,and a large number of studies have shown that the nettle polysaccharides extracted from nettle have good anti-radiation and anti-plateau hypoxia caused by cerebral edema and pulmonary edema.At present,there are also a series of related experiments,and it has been found that the extracted nettle polysaccharides have certain effects in anti-radiation damage and anti-oxidation.However,there are not many studies on the structure-activity relationship of nettle polysaccharides,because at present,the overall study of the structure-activity relationship of polysaccharides from the molecular level is relatively rare,which is a very novel research direction,which is still in the initial and exploration stage,so the research on the structure-activity relationship of polysaccharides rarely involves the molecular level,and it is impossible to elaborate the structure-activity relationship of polysaccharides from the molecular level.Therefore,this paper attempts to elucidate the structure-activity relationship of nettle polysaccharides and explore how to study the structure-activity relationship of polysaccharides from the molecular level.The specific research content is as follows:(1)The molecular weight of PAP-1 is 6.931×10~5.It is a very large polysaccharide molecule.It is very difficult to study as a whole.Therefore,through the calculation and simplification of PAP-1,the research object is determined as the PAP-1 molecule.A repeating unit pap-1 in PAP-1.(2)The pap-1 was re-expressed using the latest international unified polysaccharide notation,including IUPAC notation,WURCS notation,Glyco CT notation and SNFG notation,and the SNFG notation and the data viewed in the VMD software were re-expressed.Combined with the three-dimensional spatial structure of pap-1,the 3D drawing software was used to make its ball-and-stick model through drawing,modeling and rendering.It shows that the localized PAP-1 exhibits the shape of a single-stranded helix,including a small spiral arm composed of Glc and a large spiral arm composed of Glc and Man.In addition,the ball-and-stick model established by 3D-SNFG method can easily see the spatial structure of polysaccharide molecules,and can easily read the monosaccharide composition of polysaccharide molecules,which is of great help for the study of biological macromolecules.(3)Through molecular docking calculation,and according to Dock’s scoring principle,it is shown that pap-1 can indeed bind well to TNF-α,HIF-1α,IL-6αand VEGF proteins,and it is speculated that it forms a glycoprotein to exert its effect.It verifies the conclusions reached by the research group through animal experiments in the early stage.(4)Through the visualization data of Glycome Atlas,after observation,it is speculated that the distribution of pap-1 in mice is more extensive than that in humans.After humans eat fern polysaccharides and mice eat fern polysaccharides,they have similar effects,and can exert anti-radiation and anti-deficiency.The efficacy of pulmonary edema and cerebral edema caused by oxygen also verifies the conclusion of the previous animal experiments that the polysaccharide of fern can be anti-radiation,and speculates that in addition to the lung and brain,the polysaccharide of fern may also be distributed in the skin,liver,heart and other organs,Play the role of protecting the heart and anti-virus.In this paper,the structure-activity relationship of nettle polysaccharides and how to study the structure-activity relationship of polysaccharides from the molecular level are explored,hoping to provide reference value for the study of polysaccharide structure-activity relationship,and also provide a certain scientific basis for the development,processing and utilization of plant resources of nettle polysaccharides.