Design Of In-vivo Imaging System Based On Near Infrared Ⅱ Fluorescence

With the rapid development of Near Infrared Ⅱ(NIR-Ⅱ,900-1880nm)in vivo imaging in the biomedical field,research into the development of in vivo imaging systems is becoming a hot topic.However,current commercial products are limited by high price,large size and stable working environment.In this paper,we propose and study a Near Infrared Ⅱ in vivo imaging system device with similar performance to similar commercial products.In order to efficiently conduct in vivo biological imaging experiments,improve the integration of imaging systems,and reduce research and development costs.Based on the application scenarios of in vivo imaging systems in biomedical research and the development requirements of related instruments,the methodology is studied and designed in terms of the hardware structure of the whole system,the control methods of mechanical hardware devices,the design of the imaging optical path system,the selection and secondary development of industrial cameras,the design of the UI interface of the upper computer software and the development of functional codes.This paper mainly carries out the project research and method implementation from two aspects of hardware equipment control and software development.Firstly,at the control end of the mechanical hardware equipment,Mazelun STM32H743 is selected to complete the control of each hardware module such as time-gated imaging,automatic displacement platform,light source following system,additional light source intensity,automatic focusing,photoelectric/metal sensor,LCD.The display screen of the lower computer can display and control the working state of the hardware equipment.Then,Python+PyQt5 was used to complete the design of the human-computer interaction UI interface of the system software and the development of functional code.It realizes the control of industrial camera working parameters(exposure time,gain,etc.)and the display of its running state,realizes the regulation of hardware equipment by establishing communication with the lower computer end,and completes the secondary development and call of industrial camera through programming.In addition,the host computer system interface also includes image data acquisition,storage,display,analysis and processing,historical data query and other modules.Secondly,the function test of each module is carried out,the system is integrated and assembled,and the system function is constantly modified and improved according to the problems of the system joint adjustment.Finally,under different wavelengths of excitation light sources,a series of phantom and live animal experiments were used to collect contour images of mice.After repeated verification and testing,the performance evaluation and functional verification of the system are carried out.The realization of this system provides an instrument platform for the research of small animal in vivo imaging experiment in the field of biomedical engineering,and has certain significance.The paper concludes with an analytical summary based on the results of a comprehensive system test.