Preparation And Biomedical Applications Of Iron Manganese Silicate Nanomaterial

In recent years,nanomaterials have been widely used in various fields due to their unique properties,especially in the diagnosis,prevention,and treatment of diseases,which have shown broad application prospects and received a lot of attention from researchers.Silicate nanomaterials have unique physicochemical properties and good biocompatibility,which have been widely used in various fields such as therapeutics,catalysis,and environmental management.However,little research has been reported in vaccines and detections.In this work,we prepared an iron manganese silicate nanomaterial and explored its biomedical applications in vaccines and detections.Firstly,as an immune adjuvant,iron manganese silicate nanoparticles can slowly release manganese ions to enhance the innate immune response of the organism and achieve long-term effective immune stimulation.At the same time,they can efficiently load antigens to achieve the effect of improving the immune response of the organism and preventing plague.On this basis,we developed a biocompatible plague subunit nanovaccine,which can not only effectively promote the maturation of dendritic cells in vitro,but also induce strong responses of humoral and cellular immunity in vivo,and exhibit highly effective immune protection.As the protection rate of the pure antigenic protein group immunized alone was less than 10%,the protection rate of nanovaccine group could reach 100%within 14 days.Then,we developed a visualization technique for the rapid and highly sensitive detection of pyrophosphate content based on the peroxide-like activity and pyrophosphate responsiveness of iron manganese silicate nanoparticles.The assay has excellent sensitivity and specificity with a minimum detection limit of 205.5 nmol L^-1for pyrophosphate,and has the advantages of fast detection and simple operation.In addition,the iron manganese silicate nanoparticles are stable and can still maintain more than95%of peroxidase-like activity within 6 months.We hope that the nanovaccine and assay based on iron manganese silicate nanoparticles can promote the development of nanomaterials in practical applications and provide new ideas for the prevention and early diagnosis of diseases.