Research And Implementation Of A Microfluidic Device For Combined Multiplex Detection

Parallel combination testing of multiple targets for a variety of diseases is an important reference for efficient clinical diagnosis.For example,pre-pregnancy TORCH pathogen infection screening is performed by screening human Ig G and Ig M levels associated with five pathogens:toxoplasma,Rubella virus,Cytomegalovirus,Herpes simplex virus I,and Herpes simplex virus II,to determine the infection status of the test subject.In the heart attack test,cardiac Troponin I(c Tn I)and cardiac Troponin T(c Tn T)are tested simultaneously to determine the current myocardial damage of the subject.The commonly used methods include colloidal gold,enzyme-linked immunoassay,and chemiluminescent assay.Among them,chemiluminescence method has the characteristics of high sensitivity and high specificity.In recent years,microfluidic chip-based detection technology is gradually being promoted and applied in the field of rapid detection due to its low reagent consumption,high integration,high automation and high reaction efficiency.Combining chemiluminescence detection with microfluidics to build a new multi-indicator chemiluminescence detection system based on microfluidic chips is of great significance to improve the performance and level of field rapid detection.In this paper,this study is divided into two aspects.On the one hand,for TORCH multi-indicator detection,the enzymatic chemiluminescence method is combined with microfluidics,and a one-dimensional array of microbead microfluidic system is investigated and realized,which can simultaneously complete the screening of five TORCH indicators by a single reaction.Meanwhile,based on the integration and design of the opto-mechanical integration system,the study realized a fully automated TORCH multi-indicator assay device with automatic pipetting function,allowing four microfluidic chip channel reactions to be performed simultaneously,realizing a simple,efficient and convenient multi-indicator co-test.The performance of this multi-indicator cascade microfluidic system was tested by testing samples and verifying its key performance.On the other hand,a microfluidic system for parallel detection of two indicators was developed by combining a magnetic bead-based chemiluminescence immunoassay method with a microfluidic chip,in which multiple reaction steps related to chemiluminescence detection were completed continuously in a microfluidic chip environment,and the chemiluminescence signal was read in real time by a photomultiplier tube.Meanwhile,based on the integration and design of the opto-mechanical integration system,the research realized a fully automated chemiluminescence multi-indicator assay device with automatic pipetting function,which realized a convenient,simple and fast multi-indicator co-test.The performance of the multiplexed microfluidic system was tested by testing samples to verify its technical feasibility.