Application Of Prussian Blue Nanomaterials For Cell Membrane Camouflage In Bioanalysis

In recent years,nanotechnology has shown great potential in the field of biomedicine.Among them,cell membrane biomimetic nanomaterials have many unique advantages in nanomedicine,including improving biocompatibility,homologous targeting,extending blood circulation time and immune escape ability.Combined with the good photothermal properties and enzyme catalytic activity of Prussian blue nanoparticles(PBs),we designed and prepared a biomimetic nanocomposite that can be used for cancer treatment and cell quantitative analysis.The specific research content is as follows:1.Construction of self-actuated biomimetic nanocomposites PB/PM/HRP/Apt: This paper designed and synthesized a multifunctional therapeutic platform with unique working mechanism and good therapeutic effect on tumor.Firstly,the platelet membrane(PM)was coated with PBs as the core.As a photothermal agent(PTA)with good photothermal conversion efficiency,PBs can obtain good photothermal performance under near-infrared(NIR)light irradiation.Then,the surface of the synthesized nanocomposites was modified with horseradish peroxidase(HRP).The presence of HRP leads to the decomposition of hydrogen peroxide in the TME,thus enhancing the deep penetration of the nanocomposite into cancer cells.Finally,the modification of PD-L1 aptamer and 4T1 cell aptamer AS1411 on nanocomposites can achieve immunotherapy and enhanced targeting.The combination of photothermal therapy(PTT)and immune blocking agent can cause a strong immune response and greatly improve the tumor killing ability.Most importantly,HRP loading significantly improved the transport efficiency of the nanocomposites and promoted their aggregation at the tumor site.As a promising therapeutic strategy,this biomimetic nanoplatform sheds new light on current cancer treatment.2.Construction of a multifunctional biomimetic nanoplatform for the isolation and quantitative analysis of breast cancer cells by the combination of PB/PM/HRP/Apt nanocatalysts with good catalytic performance and functionalized microplates: Aptamers functionalized microplates have great potential in liquid biopsy due to their programmable nature.Here,we report a 4T1 cell aptamer programming strategy to precisely control the capture and release of 4T1 cells on a microplate.In addition,we developed a nano-catalyst PB/PM/HRP/Apt with good peroxidase(POD)activity.As an efficient nano-catalyst,ox TMB was rapidly generated by oxidation of H2O2 in the presence of the chromogenic agent 3,3 ’,5,5’-tetramethylbenzidine(TMB).The cells were quantified by colorimetry.As a promising therapeutic strategy,this combined detection method provides a feasible operation mode for the detection of 4T1 cells.In conclusion,this paper,based on the biomimetic nanocomposites formed by cell membrane,combined with the unique advantages of PB and other biological molecules,realized the multi-mode collaborative treatment of cancer and cell quantitative analysis,and provided a theoretical basis for the research of biomimetic nanomaterials in the biomedical field.