Interactions Between Plants And Soil Microorganisms In The Yili Tekes River Riparian Zone

Taking microbial communities,plant communities,plant functional group biomass,and soil soil environment factors in different habitats of the Yili Tekes River riparian zone as the research objects,through field investigation and sampling analysis,the composition,distribution characteristics,and important values of plant communities in different habitats of the Yili Tekes River riparian zone were investigated and analyzed.The composition,distribution,abundance characteristics,and diversity of soil bacterial communities in different habitats were analyzed using the third-generation full length sequencing technology.Redundancy analysis and correlation cluster heat map analysis were used to explore the relationship between microbial communities and soil environment factors,as well as the relationship between plant communities and plant functional group biomass,and soil environment factors,revealing the interaction and interaction among ‘plant-soil environment factors-soil microorganisms’.Provide basic information for the subsequent protection,restoration,and sustainable development of plant species in Yili Tekes River Wetland.The main research results are as follows:1.After sequencing 9 soil samples,a total of 65812 CCS sequences were obtained through Barcode identification,divided into 8654 OTUs,and the coverage of sequencing was greater than 97%.A total of 36 bacterial phyla,94 classes,296 orders,622 families,1110 genera,and 1688 species were detected in the soil.Proteobacteria,Acidobacillus,and Bacteroidota account for 56.67% of the total sequence,and these microbial communities are dominant.2.In diversity analysis,The α-diversity index analysis shows that the ACE index and Chao1 index of riverbank habitat R are both greater than those of flood plain F,and those of flood plain F are greater than those of terrace T.The Shannon index and Simpson index of habitat R and habitat F have almost no difference and are greater than those of habitat T.In β-diversity analysis,T1,T2,and T3 have high similarity,R2,R3,and F3 have high similarity,F1,and F2 have high similarity,and R1 samples have poor similarity with other samples.3.A total of 76 species of plants were observed and recorded in the study area,belonging to 28 families and 63 genera.Among them,there are 13 species of Compositae plants.The species with the highest important value in terrace habitats is Carex turkestanica,with a maximum value of 18.83%.The second is Caragana aurantiaca(16.00%).The species with the highest important value in the floodplain habitat is the Inula britanica,with a maximum value of 12.55%.The second is Caragana aurantiaca,accounting for 10.70%.The species with the highest important value in riparian habitats is the Inula britanica,with a maximum value of 12.24%.The second is Carex turkestanica,accounting for 11.00%.4.The redundancy analysis and correlation clustering heatmap analysis of microbial communities and soil environmental factors showed that the correlation between soil environmental factors and the horizontal distribution of soil microbial bacterial communities was as follows: total phosphorus(TP)>organic matter(SOM)>available phosphorus(AP)>soil moisture(SM)>total nitrogen(TN)>available nitrogen(AN)>pH.SOM and TP have the most similar effects on bacteria;SM and pH have similar effects on bacteria;The effects of AN and TN on bacteria are relatively similar;Poor similarity between AP and other environmental factors.5.Redundancy analysis and correlation cluster heat map analysis were cond ucted on the biomass of plant communities,environmental factors,and plant fu nctional groups.The results showed that pH and total biomass(total)were t he main influencing factors on plant communities(p=0.005,p=0.007),followed by forb biomass(forb)and grass biomass(grass)(p=0.030,p=0.028).The corr elation between soil environmental factors and functional group biomass and pl ant community distribution was pH>total>grass>forb>>SOM>TP>AP>SM>sedg e>AN>TN.6.The main path of interaction between soil bacterial community and plant community is: plant richness→AP→bacterial diversity.As plant richness increa ses,the content of available phosphorus in soil increases,which in turn leads t o an increase in bacterial diversity.