A Preliminary Study On The Function Of RRP15 In DNA Damage Repai

DNA in human cells is affected by the external environment and internal metabolism,and various damages may occur,such as single-strand breaks,double-strand breaks,base mismatch,DNA interstrand cross-linking,etc.If these damages are not repaired in time,they can lead to cell death or cancer-causing mutations.Therefore,DNA damage repair is an important guarantee for maintaining the stability of the cell genome.DNA damage repair response is one of the most important processes in living organisms,consisting of a variety of different mechanisms,including direct repair,base excision repair,nucleotide resection repair,mismatch repair,etc.These mechanisms allow cells to detect and repair DNA damage,maintaining genome integrity and stability.If the DNA damage repair response mechanism is faulty,it can lead to diseases such as genetic diseases,immunodeficiency diseases,neurological diseases,and cancer.Therefore,the study of the mechanism of the DNA damage repair response is essential for the development of methods and preventive measures to treat these diseases.Recent studies have shown that RRP15(RNA processing protein 15,also called CGI11 5)plays an important role in a variety of biological processes,which can not only maintain the integrity of the nucleolar structure,but also promote the process of ribosome biogenesis and cell proliferation regulation.However,it is unclear whether RRP1 5 is involved in the DNA damage response and the role of RRP1 5 in tumor development and development.Therefore,this study uses cellular and molecular biology techniques to further explore the role of RRP15 in DNA damage repair response and the mechanism by which it may be involved in tumorigenesis and development.By constructing a recombinant plasmid with GFP tag,and then using laser to induce DNA damage in living cells,it was found that RRP1 5 protein with GFP tag could be recruited to DNA damage sites relatively quickly through multidimensional image acquisition and analysis,and the recruitment of RRP1 5 at DNA damage sites was further confirmed by immunofluorescence experiments.This indicates that RRP15 can be involved in DNA damage repair.In order to better understand the specific mechanism of action of RRP15 in this process,we will treat cells knocked down by RRP15-siRNA with chemotherapy drugs,preliminarily determine which chemotherapy drugs are mainly involved in the DNA damage response induced by RRP15,and found that the resistance to the chemotherapy drug cisplatin in the experimental group was significantly higher than that of wild-type cells.Next,from the protein immunoimprinting experiment,we found that the protein level of intracellular RRP1 5 increased significantly after treating cells with cisplatin,which indicates that cisplatin can induce an increase in intracellular RRP1 5 protein,thereby participating in DNA damage repair.At the same time,it was found that RRP15-siRNA knocked down cells led to an increase in apoptosis.This finding suggests that RRP1 5 may play an important role in regulating cellular life activities and help us better understand the mechanisms of apoptosis.In summary,this study clarifies the involvement of RRP1 5 in DNA damage response,preliminarily reveals the correlation between RRP15 and cisplatin-induced DNA damage repair,and finds that RRP1 5 downregulation leads to apoptosis,which provides a certain basis for in-depth exploration of the role of RRP15 in DNA damage repair and tumorigenesis and development,and also provides a certain reference for the future development of related treatments and preventive measures.