Detection Of Drug Resistance And Analysis Of Related Drug Resistance Genes Of Escherichia Coli From Different Animal Sources In Zhaosu,Ili

In order to understand drug resistance and carrying of drug resistance gene in Escherichia coli from different animal sources in Zhaosu area of Ili,Xinjiang and give drug guidance pertinently to corresponding villages.Cattle nose and anal swabs（200 samples both）were collected from twelve sampling points such as Sitas village,Uklegar village and Simutas village in Zhaosu area of Ili;sheep nose and anal swabs（210samples both）were collected from four sampling points in Simutas village,Bedie village,Sentas village family and Sentas village breeding community;chicken cloacal swabs（300 samples）were collected from three sampling points:Kemakgar village,Sentas village breeding community and Uklegar village;equine nose and anal swabs（46 samples each）were collected from farmers in Kashagar town,which was 1212samples in total to conduct isolation and identification of Escherichia coli.The MIC values of six categories and 10 kinds of antibiotics were determined by agar dilution method,and the corresponding drug resistance genes were detected by PCR.The results are as follows:1.Results of Escherichia coli isolation:1010 strains of Escherichia coli were isolated from 1212samples,the separation rate was 83.3%.The separation rate of Escherichia coli in various samples were as followed:Cattle anal swab（96.5%,193/200）,sheep anal swab（93.3%,196/210）,chicken cloacal swab（96.3%,289/300）,equine anal swab（91.3%,42/46）;cattle nose swab（53.5%,107/200）,sheep nose swab（77.1%,162/210）,equine nose swab（45.7%,21/46）.2.Results of drug resistance phenotype test:The resistance rate of Escherichia coli in Zhaosu,Ili to ampicillin was the highest,which was 10.1%（102/1010）,and the drug resistance rates of other tested antibiotics were less than 10.0%.（1）Multidrug resistances of 33 strains of Escherichia coli from cattle were mainly distributed between 1～3,among which one resistance was main multidrug resistance,accounting for 70.0%（23/33）.Only one strain of one resistance Escherichia coli was isolated from cattle in Uklegar village and Ye letai village respectively,and the drug resistance rates were 14.3%（1/7）and 6.3%（1/16）.Escherichia coli from cattle in Sittas village,Sentas village pasture,Sentas village breeding community and Sentas village family was one resistance mainly,accounting for 75.0%（9/12）,75.0%（6/8）,75.0%（6/8）and 66.7%（2/3）.No drug resistance strains were isolated from other sampling points.（2）Escherichia coli from sheep was sensitive to imipenem,amikacin and colistin sulfate.The drug resistance rates of all sampling points to the rest of tested antibiotics were less than 3.0%.（3）Escherichia coli from chicken was resistant to ampicillin（31.5%,91/289）,florfenicol（26.0%,75/289）and tetracycline（20.1%,58/289）,of which resistance rate was the highest,greater than 20.0%.The drug resistance rates of Escherichia coli from chicken in Senas village to ampicillin（40.0%,8/20）,cefazolin（35.0%,7/20）,fluorbennetic（45.0%,9/20）,enrofloxacin（40.0%,8/20）,colistin sulfate（30.0%,6/20）were 30.0%or more.Escherichia coli from chicken in Kamgar village is the most abundant,reaching 32 species.The drug resistance rates of Escherichia coli from chicken in Ukregal village were the lowest,all of which were less than 20.0%.The drug resistance severity of Escherichia coli from chicken was sequentially followed by Sentas village,Kamakar village and Ukregal village.3.The detection rates of drug resistance genes in Escherichia coli from different animal sources in Ili,Zhaosu were sequentially followed by chicken,sheep,cattle and equine.The drug resistance gene detection rates of Escherichia coli from cattle and sheep were extremely low,the drug resistance gene detection rate of Escherichia coli from chicken was a little low.bla_CTX-M gene（0.3%,1/300）was detected in a strain of Escherichia coli from cattle in Sentas village.bla _TEM gene（0.3%,1/358）,flo R gene（0.3%,1/358）and tet A gene（0.3%,1/358）were detected in a strain of Escherichia coli from sheep in Simutas village at the same time.Escherichia coli from chicken detected nine drug resistance genes,among which flo R gene（25.6%,74/289）,tet A gene（18.0%,52/289）and bla _TEM gene（18.0%,52/289）were detected in more than 17.0%.They were also genes with the highest detection rates of Escherichia coli from chicken in Kamakar village.Of all drug resistance genes detected,Escherichia coli from chicken in Sentas village was only detected four genes that were tet A gene（20.0%,4/20）,flo R gene（50.0%,10/20）,bla _CTX-M gene（15.0%,3/20）and bla _TEM gene（20.0%,4/20）.However,the carrying rates were high.In addition to the flo R gene detection rate of 18.7%,the detection rates of the other five drug resistance genes of Escherichia coli from chicken in Uklegar village were between 1.3%～6.7%.Conclusion:There are differences in drug resistance among different animal sources in Ili,Zhaosu.Except Escherichia coli from equine,there were multidrug resistant strains that were resistant to three or more antibiotics at the same time in Escherichia coli from other animals.The drug resistance of Escherichia coli in different sampling points were also different.Antibiotics should be selected accordingly in case further diffusion of drug resistance bacteria carrying drug resistance genes.