The Mining Of Probiotics For Repairing Hair Damage Efficacy Based On The Multi-omics

Alopecia is one of the health problems faced by middle-aged and elderly people in the world,and it has gradually become younger in recent years.With the deepening studying scalp microbiota,Repair hair loss with probiotics has become a new hot spot in scientific research and market application,but the mechanism of probiotics to repair alopecia is not clear enough.Hence the probiotics in repairing hair damage was analyzed through multi-omics combination in this research.Firstly,the composition of scalp microbiome and hair loss in hair loss patients and normal people were analyzed.Then,the differential bacterials were isolated from scalp samples by directional separation technology,and the characteristics of these bacterials were progressively analyzed.Next,by establishing a hair loss damage model of hair follicle papilla cells,300 probiotics isolated from healthy human feces,fermentative foods and other samples were used as research candidates,and Lactobacillus plantarum L30 and Bifidobacterium bifidobacteria 070310 were targeted and screened.From the disorders of probiotics regulating the balance of scalp microecology,improving damaged hair follicle papilla cells and regulating scalp androgen levels,multi-omics(microbiome+genomics+metabolomics+enzymology)were used to explore the mechanism of action and related pathways for repairing hair damage,which lay a theoretical foundation for the development of safe and effective foods and drugs.The main findings and conclusions are as follows:1)16S rRNA gene high-throughput sequencing technology was used to study the scalp and hair follicle microecology of hair loss patients and normal people.The results showed that there was a significant differences between hair loss population and the normal population in the composition of scalp microorganisms and hair loss would lead to a downward regulation of the diversity and abundance of human scalp microbial diversity,while the abundance of conditional pathogenic bacteria such as Propionibacterium acnes,Staphylococcus aureus and pigment-producing micrococcus were up-regulated,and there was a significant difference in the abundance of Propionibacterium acnes in hair follicles of hair loss patients.2)Microbial separation technology was used to isolate and culture microorganisms in scalp and hair follicle samples of hair loss patients and normal people,and the Key microorganisms were analyzed by whole genome sequencing.The results showed that the scalp microorganisms in normal population mainly including Staphylococcus epidermidis,Staphylococcus cephalis and a small number of Staphylococcus aureus.The abundance of Staphylococcus epidermidis and Staphylococcus cephalis was significantly reduced in the scalp of hair loss patients,and the abundance of Bacillus,Staphylococcus aureus and pigment-producing micrococcus was significantly regulated.In addition,Propionibacterium acnes was not isolated from scalp samples,but was successfully isolated from hair follicle samples.3)High performance liquid chromatography-mass spectrometry(LC-MS)was used to study the effect of Propionibacterium acnes on the metabolic level of hair follicle papilla cells.The results showed that the contents of B vitamins such as niacinamide,riboflavin,thiamine,D panthenol,biotin,glutamine and cysteine in hair follicle papilla cells were significantly downregulated after the intervention of Propionibacterium acnes.4)A hair loss damage model of hair follicle cells was established by DHT,and 300 strain of probiotics were isolated from healthy human feces,food,air and soil samples from Tibet,Beijing and Guangzhou,and the functional characteristics of these strains were studied by RT-PCR and LC-MS.The results showed that the isolated probiotics mainly included Lactobacillus plantarum,Lactobacillus reuteri and Bifidobacterium.Lactobacillus plantarum L30 and Bifidobacterium bifidobacteria 0 70310 were obtained by targeted screening and RT-PCR results showed that L30 and 070310 could significantly upregulated the gene levels of VEGF and IGF-1.The results of LC-MS analysis showed that L30 and 070310 could significantly repair the damage of cells after DHT action,and upregulated the content of micronutrients such as niacinamide,biotin and glutamine.5)The protein associated with hair loss(5a reductase)was obtained by in vitro heterologous expression,and its protein activity and the inhibitory effect of Lactobacillus plantarum L30 and Bifidobacterium bifidobacteria 070310 were detected.The results showed that L30 and 070310 could significantly inhibit 5αreductase activity,and regulate the level of scalp androgens indirectly and improve hair loss.In summary,the probiotics of Lactobacillus plantarum L30 and Bifidobacterium bifidobacteria 070310,can regulate the balance of scalp microecology and scalp androgens,and can also improve the micronutrients deficiency and hair growth factor levels caused by androgenetic alopecia,thereby repairing hair damage,which laying a theoretical foundation for the development of safer and more effective foods and drugs.