Minocycline Microfluidic Detection Based On Hollow PMMA/PS Polymer Fiber

A low-cost hollow PMMA/PS polymer optical fiber microfluidic device was prepared in this study.The miniaturization of the microfluidic device could be achieved by using optical fibers.Low melting point and high flexibility of the polymer were used to reduce the difficulty of preparation.By combining the advantages of the chemiluminescence method such as simple equipment and freedom from background light interference,the determination of minocycline in human serum environment was achieved.Online coupling and transmission of signal light were accomplished through the interaction between the signal light in solution and the suspension core.Compared with the traditional method,the design is low-cost and short cycle time,and realizes the real-time online detection function of minocycline.The main work can be summarized as follows:1.The hollow PMMA/PS polymer fiber preforms were prepared.The effects of drawing tower parameters on the stretching of the fiber preforms were investigated,and the optimum stretching temperature of 180 ℃ and traction speed of 0.66 m/min were obtained.The overall outer diameter of the hollow polymer fiber is about 950±30 μm,the wall thickness of the cladding is about 200 μm,and the diameter of the suspended core is about 190～200 μm.Two suitable microfluidic channel construction methods for polymer fibers are introduced:pneumatic opening method and local hot melt opening method.The microfluidic channel construction was completed using the local hot-melt opening method.2.The hollow PMMA/PS polymer optical fiber microfluidic device was initially tested to demonstrate its ability to perform long-period online detection with good reproducibility.The optimal reaction conditions were obtained by optimization experiments: Na OH concentration0.1 mol/L,luminol concentration 0.01 mol/L,and potassium ferricyanide concentration 0.005mol/L.Under the optimal conditions,the detection limit of minocycline was 0.1～0.0001 g/L and the linear range was 0.001～0.01 g/L for the hollow polymer fiber optic microfluidic device,which was resistant to interference.The two minocycline concentration responses,0.001 g/L and 0.002 g/L,were tested in human serum environment with maximum deviations of 3.2%and 2.4%,respectively.It indicates that the device has great potential for medical and biosensing fields.