Effects Of Neuronal Information Transmission By Focused Ultrasound Based On EPSP And LFP

The hippocampus of mammalian brain is a region with high concentration of neurons.If the neurons in this region are abnormal,it will often lead to neurodegenerative diseases.The analysis and processing of neuroelectric signals is a very important part of the treatment of neurodegenerative diseases.However,the current ultrasonic stimulation experiments lack the host computer control software with adjustable ultrasonic parameters and electrophysiological data.Therefore,combined with the existing ultrasonic nerve stimulation system,this paper designed the upper computer control software,built the ultrasonic stimulation experimental platform,and analyzed the data of excitatory postsynaptic potential and local field potential to explore the regulatory effect of lowintensity focused ultrasound on rat hippocampus.Firstly,an upper computer control software with adjustable ultrasonic parameters was designed and an experimental platform of ultrasonic stimulation was built.According to the characteristics of the ultrasonic nerve stimulation system and the basic requirements of the experiment,the main function modules of the software were designed.The software consists of three control panels,which are stimulus control,parameter setting and object list.It can control the stimulus state,select the trigger mode,read the stimulus time,modify and store the ultrasonic parameters.In this paper,the intensity of sound field was measured and the index was tested on the ultrasonic stimulation platform,so as to determine the ultrasonic parameters and stimulation Angle used in the experiment.Secondly,to investigate whether low-intensity focused ultrasound stimulation can affect the information transmission of neurons in isolated brain tissues,we hypothesized that low-intensity focused ultrasound stimulation can regulate the information transmission of hippocampal neurons after obtaining excitatory postsynaptic potential signals.After obtaining excitatory postsynaptic potential data stimulated by different ultrasonic intensities,the data were preprocessed to remove clutter and noise interference,and then analyzed from time domain and nonlinear dynamics.It was found that low intensity focused ultrasound could inhibit the excitability of hippocampus neurons in isolated brain slices.Finally,aiming at the problem of whether low-intensity focused ultrasound stimulation can affect the information transmission of biological neurons in vivo,we propose the hypothesis that low-intensity focused ultrasound stimulation can regulate the information transmission of hippocampal neurons after obtaining local field potential signals.After obtaining the local field potential signal,the signal was filtered and denoised,and then analyzed from the time domain,frequency domain and nonlinear dynamics.It was found that low-intensity focused ultrasound can enhance the excitability of living rat hippocampal neurons.