Preliminary Studys On The Function Of Two Vascular Development Related LRR-RLK Genes In Arabidopsis

Plant vascular system,as a distinctive feature from algae plant era to pteridophytes plant era,is important for plant growth and development.On one hand,it is involved in material transporting in plants;on the other hand,it also has a function of supporting itself.In the development process of the plant vascular system,the peptide hormone and its receptor protein kinase used for intercellular signal transferring mechanisms determine the direction of the vascular system development.The genes ATVC2 and PXY belong to the LRR-RLK gene family closed to plant vascular development,whose function research focused on the field of plant vascular system.The Arabidopsis gene ATVC2 in this dissertation,as a receptor of CLV3 peptide,whose function is not yet clear.In addition to the antisense gene ATVC2-NAT1 from the TAIR website,some other antisense transcripts were found by the chain-specific RT-PCR.In order to study these antisense transcripts,the paper obtains these antisense transcripts’ ends by RACE.In the study of ATVC2 function,the insertion mutants N53 and N48 were screened for genome and validated for expression;the ATVC2 overexpression lines,ATVC2-NAT1 overexpression lines and RNAi lines were also screened and validated.To our surprise,the ATVC2 expression level was not changed in these several lines.Another study in this paper is PXY in Arabidopsis.As the TDIF receptor,PXY is related to cambium cell division and differentiation.The purpose of the experiment is to study the evolution relationship between the gene PXY in Arabidopsis and the gene PXY-LIKE in Selaginella.The Arabidopsis PXY’s promoter and the Selaginella PXY-LIKE’s coding region were combined into a new sequence by the recombinant PCR method.Then we transferred the new sequence into the Arabidopsis mutants N526128.Finally,we will observe the phenotype reply whether or not to study the evolution relationship.