| Sclerotinia sclerotiorum is a necrotrophic plant pathogenic fungus,which can obtain nutrition by inducing host plant necrosis.The necrosis of host plant tissue is caused by the increase of reactive oxygen species at the site of infection and apoptosis.In the process of infection,Sclerotinia sclerotiorum can exert the ability of scavenging reactive oxygen species to resist host induced oxidative damage.In this study,the gene functions of glutathione peroxidase and reductase coding genes in Sclerotinia sclerotiorum were analyzed by gene function analysis,in order to further explore the pathogenic mechanism of Sclerotinia sclerotiorum.The main results are as follows:(1)Susceptibility of Sclerotinia sclerotiorum to metal ions.The results showed that the growth and development of Sclerotinia sclerotiorum were disturbed with the increase of metal ion concentration.The specific performance is that the mycelium grows slowly,the number of sclerotia decreases,and ferrous ions,zinc ions and copper ions did not produce sclerotia at the concentrations of 8 mm,8 mm,20 mm and 10 mm respectively.The results showed that different metal ions affected the mycelial growth and sclerotia development of Sclerotinia sclerotiorum.(2)Functional analysis of SsGP1 gene in Sclerotinia sclerotiorum.Bioinformatics analysis showed that SsGP1 gene had high homology between Sclerotinia sclerotiorum and Botrytis cinerea.In the process of infection,the expression of SsGP1 gene in Sclerotinia sclerotiorum increased gradually,and increased significantly in the later stage of infection.Through the analysis of gene deletion and mutation,the deletion of SsGP1 gene in Sclerotinia sclerotiorum led to the increase of the branching angle of mycelium tip and the slow growth of colony.It is speculated that SsGP1 gene is involved in the growth and development of Sclerotinia sclerotiorum.(2)Functional analysis of SsGR1 gene in Sclerotinia sclerotiorum.Bioinformatics analysis showed that SsGR1 gene in Sclerotinia sclerotiorum had high homology in Botrytis cinerea.In the process of infection,the expression of SsGR1 gene in Sclerotinia sclerotiorum increased gradually,and increased significantly in the later stage of infection.Through the study of gene deletion,it was found that the deletion of SsGR1 gene in Sclerotinia sclerotiorum did not affect mycelial growth,mycelial branching and Sclerotia formation;Under copper ion stress,the mutant showed slow mycelial growth.It is inferred that SsGR1 gene is involved in the stress resistance regulation of Sclerotinia sclerotiorum to copper ion.This study explored the function of glutathione peroxidase and reductase coding genes of Sclerotinia sclerotiorum,so as to provide scientific reference for exploring the growth and development of Sclerotinia sclerotiorum and the regulation of pathogenic molecules. |
