| The natural carbon cycle process is persecuted due to frequent human activities,causing a series of current environmental problems.The reservoir ecosystem is an important part of the natural carbon cycle.In order to further understand the natural carbon cycle in the reservoir ecosystem,this study selected The main urban section of the Yangtze River in Chongqing is the main research object,to study the basic water quality indicators of the water body and the content of various forms of carbon in the water body and sediment sediments in the basin and typical tributaries(Jialing River).Changes in distribution,key ways of CH4 production and main sources of CO2in water;indoor Carbon,Nitrogen concentration variation parameter simulation experiments using sediments,and analysis of different concentrations of Carbon and Nitrogen sources combined with changes in water quality indicators and carbon stable isotopes during the experiment Inputting the relationship between the production and sinking of sediment,CH4 and CO2in the background,the following conclusions are obtained:(1)The variation range of CH4 concentration in the water body in the study area and time period is 0.099μmol/L to 0.144μmol/L,the CH4 stable carbon isotope in the water body ranges from-43.25‰to-50.14‰;the variation range of the CO2concentration in the water body is 23.051μmol/L~30.143μmol/L,the stable carbon isotope of CO2fluctuates greatly in the water body,and the fluctuation range is-11.07‰~-14.97‰;the downstream water body is obviously affected by the upstream water body transfer,and the Tangjiatuo and Guojiatuo points in the middle and lower reaches of the main stream are affected by external sources.The influence of C and N input is more obvious.The concentrations of CH4 and CO2and their carbon isotopes are generally higher than those of the upstream point,and the methane production intensity is stronger.(2)The key pathways of CH4 production are mainly acetic acid fermentation,the proportion of which fluctuates from 84%to 97%along the way,and the average is90.6%.The proportion of acetic acid fermentation at the tributary point is(97%)was significantly higher than that of the mainstream point;the source analysis of CO2in water mainly considered three situations:carbonate weathering,substrate organic matter decomposition,and terrigenous input.(3)The concentration of CO2 in the water body has a significant positive correlation with dissolved oxygen DO,dissolved inorganic carbon DIC,and nitrous nitrogen NO2--N;CH4 carbon isotopeδ13C-CH4 has a significant positive correlation with dissolved oxygen DO;while CO2carbon The isotopeδ13C-CO2 was significantly negatively correlated with dissolved oxygen DO.(4)Exogenous Carbon(C)input will promote the production of CH4 in the sediment,and reduce the oxidation rate of methane,so that the concentration of CH4 increases;the higher the concentration of C source input,the higher the production rate of CH4 ,and the methanogens in the 8th The hourly activity was the highest,and the yield reached the peak value;C1(10 mg/L)concentration of carbon source input was the most suitable for bacterial growth,and methanogens had the highest methane production rate.The input of exogenous C will make the respiration of microorganisms in the water body more obvious,and the decomposition of organic matter will produce more CO2;the higher the concentration of C source input will make the final concentration of CO2in the water body higher,the C source input of C1(10 mg/L)concentration will affect CO2.The impact of the output and sink is more obvious;the higher concentration of C source input will make the final overall CH4 and CO2concentration in the water body higher.(5)Exogenous nitrogen(N)input significantly improved the activity of functional bacteria,so the concentration of CH4 would increase significantly,and the C/N value and inorganic nutrient environment in the water body and sediment were changed,resulting in changes in the number of biological populations in the sediment;low concentrations The N source input showed a more obvious effect on the CH4 production and sink 6 hours before the reaction;the N1(1 mg/L)concentration nitrogen source input was more suitable for functional bacteria to play an advantage in the core stage of the reaction;the high concentration N source input made the water CO2The production and consumption mechanism of slough becomes more complex,and the CO2concentration in the water body fluctuates continuously,and the impact on the reproduction,respiration,functional bacterial community and quantity of sediment microorganisms,and CH4 and CO2sinks lasts for a longer time. |