Mechanisms Involved In Lytic And Lysogenic Regulation Of Bacteriophage VMY 22 Against Bacillus Mycoides 41-22

Bacteria-phage is an ideal model to understand the interaction between virus and host effectively.In this study,Bacillus mycoides 41-22 and its phage of VMY22 were used as model to study the mechanisms involved in lysis-lysogen regulation by new signal molecule.In this study,it was found that when 41-22 was infected by VMY 22,a kind of small molecular that could significantly affect the lysogenic decision of VMY 22 can be accumulated in the culture medium.The optimal colletion of signal molecules was determined to be 72 h at 28℃ cultivation.It has been proved that those signal molecules could be inactivated by treatment with protease K,which suggests the signal molecule should be a protein or peptide.Further studies showed that the signal molecule has a significant dose-dependent in lysogenic regulation.The integration of whole genomic DNA of VMY22 into host 41-22 by signal molecule has been identifed by PCR and other techniques,and the transformation efficiency was87%.The host cell of Bacillus 41-22 integrated by VMY 22 obtained significant resistance against infection from VMY 22.In order to explore the mechanisms of the signal molecules involved in lysis-lysogen regulation,the potential signal molecules were enriched by fractional ultrafiltration.The characters of signal molecules were analyzed and identifed by LC-MS/MS based on whole genomic datas of 41-22 and VMY 22.The results revealed that several new signal molecules with sequences of TFDAQQGDGETK,VDKHETIVKETTL and NVDKHETIVKETTL have been identifed.Compared with the published signal peptide,we found those signal molecules shuold be a new kind of signal molecules,which may involved in a new kind of lysis-lysogenic regulation mechanisms.In this paper,a new kind of polypeptide signaling molecule was found involved in lysogen-lytic regulation.The mechanisms involved in this new kind signal molecule will provide new knowledge for us to better explore the law of bacteria-phage interaction.The theoretical results can also provide reference for the treatment and prevention of human virus infection in future.