Bioinformatics Analysis Of Potential Pathogenic Genes Of Endometriosis Complicated With Infertility

Objective: The purpose of this study was to screen differentially expressed genes of endometriosis(abbreviated as endometriosis),screen differentially expressed genes of endometriosis with infertility,explore the function of common differentially expressed genes,and search for key gene(hubgene),so as to provide biomarker for clinical diagnosis and provide a new idea for further understanding the molecular mechanism of endometriosis with infertility.Methods: 1.Using "Endometriosis" as the key word,we searched the expression profile chip data in GEO(Gene Expression Omnibus)database,and selected from human tissue;Chip for whole genome RNA expression;Pairing chip for normal people(without infertility)with and without endometriosis;Pairing chip with and without infertility in endometriosis.2.By analyzing and calculating in R language,the differentially expressed gene sets of two groups with and without endometriosis in the normal population(without infertility)were screened according to the difference multiple(|log2(Fold Change)|>1)and significant level(Pvalue<0.05).That is,the gene set that may cause endometriosis in normal people.Screening the differential gene sets of two populations with and without infertility among the people suffering from endometriosis,that is,the gene sets that may lead to infertility in patients with endometriosis.The above two differentially expressed gene sets were intersected to obtain a common differentially expressed gene set,that is,a gene set that may cause endometriosis in normal people and infertility in endometriosis patients.Then Gene Ontology functional enrichment analysis and KEGG biological pathway enrichment analysis of differentially expressed genes were carried out.Finally,the protein-protein interaction network of differentially expressed genes were constructed by String database,and the hub-genes were screened by cyto Hubba plug-in in Cytoscape software.Results: 1.There were 71 differentially expressed genes,of which 11 differentially expressed genes were generally up-regulated,and 60 differentially expressed genes were generally down-regulated.2.GO functional annotation analysis showed that the differential expression genes were mainly enriched in the biological process in the actin filament organization,actomyosin structure organization,in utero embryonic development,positive regulation of L-gulamate import across plasma membrane,T-cell proliferation.In molecular functions,it was mainly involved in actin binding,carbohydrate binding,haptoglobin binding,monosaccharide binding,transferase activity,transferring alkyl or aryl(other than methyl)groups.In cellular components,the differential expression genes were mainly enriched in endocytic vesicle,endocytic vesicle lumen,endoplastic reticulum lumen,late endosome,late endosome membrane.3.KEGG pathway analysis showed that these differential expression genes were mainly enriched in 4 signaling pathways.These signal pathways include pathogenic escherichia coli infection,phagosome,protein processing in endoplasmic reticulum,cell adhesion molecules.4.The protein-protein interaction network of differentially expressed genes were constructed by String database,and the Cyto Hubba plug-in of Cytoscape software calculates hub-genes through Degree algorithm.It was found that MATN3,APOE,VCAN,PDIA6,SCG2,GOLM1,HSP90B1,GRN,TUBB,ITGB1 were most likely to act as hub-genes.Conclusion: It is suggested that MATN3,APOE,VCAN,PDIA6,SCG2,GOLM1,HSP90B1,GRN,TUBB and ITGB1 are most likely to act as hub-genes,which may play an important role in the occurrence of endometriosis and infertility in endometriosis patients.It may be used as a biomarker(Biomaker)to guide clinical diagnosis.