The Role Of Psrc1 In The Meiosis Of Mouse Oocytes

The proper assembly and migration of the spindle and the correct division of the chromosomes play a crucial role in the quality of the oocyte.In somatic cells,Psrc1 regulates spindle dynamics and mitotic progression,but its functions in oocyte meiosis have not been fully elucidated.In the current study,we demonstrated for the first time that Psrcl localizes at the spindle poles during all stages of mouse oocyte development.The loss of Psrc1 causes abnormal spindle morphology,continuous activation of spindle assembly checkpoint(SAC)protein BubR1,abnormal kinetochore-microtubule(K-M)attachments,and increases the rates of aneuploidy and large polar body extrusion.Surprisingly,either the deletion or overexpression of Psrcl tends to cause abnormal spindle assembly and increased rate of large polar body extrusion.The oocyte phenotype caused by the deletion of Asb7 is similar to that caused by Psrcl knockout.More interestingly,both proteins regulate the abundance of Kif2A.Taken together,our data suggest Psrc1 plays an important role in spindle assembly and chromosome division during mouse oocyte maturation.