Research On Human Embryonic Stem Cells Lab-on-a-chip Culture Technology

Lab-on-a-chip is a technology that uses controlled microflow to implement experiments on a chip.The 20 years of development of the lab-on-a-chip has shown great potential in various research fields.However,due to the complex manufacturing process of chips and control equipment,high cost,large size,short life span,and inconvenience,this technology cannot enter daily life like mobile phones.This paper establishes the microfluidic drive theory and finds the way to simplify the microfluidic pump process in the framework of this theory.This technique overcomes the main engineering difficulties in microfluidic chip control,and based on this technique,a magnetic oscillating pump(O-pump)is produced,which has stable performance,the size is less than 1.5 mm,and the power consumption is about 10 mW,such a low power consumption is equivalent to a removable power supply that can be used for 200 days,and the phone can be directly driven.The control signal is written into the audio file through music technology,which can be directly programmed modularly through the MP3 or music playback menu of the phone.This drive method is material independent and can be applied to any hard chip,including silicon-based allglass chips or silicon wafers.Based on the magnetic oscillation pump,an artificial cell nest circulating chip with suitable conditions for human embryonic stem cell survival is designed with a precise fluidic microenvironment control that can bring nutrients from outside the artificial cell nest into the cell nest within the stem cell shear force threshold to enhance the rate of material exchange and promote the proliferation and division of human embryonic stem cells.Combined with Matrigel matrix gel overlay method,it achieves threedimensional cell culture on artificial cell nest microfluidic chip,which makes the in vitro simulated microenvironment closer to that inside the organism.Microfluidic chip is a novel and convenient technology and research method for cell or tissue microenvironment simulation,which achieves the functions of a chip lab with the operation and cost of a traditional culture dish.