| The WRKY transcription factors,one of the largest families of transcription factors,which involve growth and development,metabolism and stress response in plants.Whether there are WRKY family transcription factors involved in the regulation of flavonoid synthesis in Camellia sinensis has not been reported.In this study,we screened and identificated the WRKY family genes from Camellia sinensis genome.Subsequently,the expression of genes under abiotic and biotic stress were analyzed,such as drought,low temperature,salt stress,and methyljasmonate treatment.Furthermore,the analysis of expression levels in different organs were performed.Based on the analysis of transcriptome and genome,62 CsWRKY genes were identified by screening and de-redundancing.Accroding to the number of WRKY domains and the type of zinc-finger-like motifs,WRKY proteins have been mainly classified into three groups: Ⅰ,Ⅱ,Ⅲ and five subgroups(Ⅱa-Ⅱe).Most WRKY transcription factors belong to group II.Among the 62 CsWRKY genes,all CsWRKY in tea plants contain introns,and most of them have 2-5 introns.The transcriptome analysis indicated that most of CsWRKY genes responded to drought,low temperature,and methyl jasmonate treatment.Through homology analysis,we predicted that CsWRKY60 and CsWRKY56 involved in regulating the biosynthesis of flavonoid in tea plant.In addition,the results of genetic complementation experiment showed that CsWRKY56 obviously rescued the accumulation of anthocyanin and proanthocyanidin of the ttg2 mutant,as well as the formaion of trichomes.CsWRKY60 partially rescued the accumulation of anthocyanin and proanthocyanidin of the ttg2 mutant.However,CsWRKY60 couldn’t restore the trichome formation of the mutant.The correlation analysis showed that the expression of CsWRKY56 and CsWRKY60 were positively correlated with the accumulation of catechins and anthocyanins in tender leaves of tea plants,respectively.In addition,CsANRb was predicted to be the target gene of CsWRKY56 and CsWRKY60. |
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