| β-glucuronidase is a member of the lysosomal glycosidase family and is mainly involved in the degradation of prokaryotic and eukaryotic glycosaminoglycans to glucuronic acid.Among various malignant tumors,β-Glu activity is higher than that of normal tissues in liver cancer,colon cancer,prostate cancer and kidney cancer,especially in necrotic areas.Therefore,β-Glu is considered a tumor biomarker.In addition,humanβ-Glu levels are also significantly elevated in many physiological diseases.Therefore,it is of great significance to develop an AIE-based probe for real-time endogenous β-Glu detection in the diagnosis and treatment of various diseases.In this paper,based on the activity characteristics of β-Glu,we constructed a fluorescent probe using Aggregation-Induced Emission material as the core of the fluorescent probe.We designed and synthesized three fluorescent probes.Probe-1 could be cleaved by β-Glu because of the low polarity of tetraphenylethene,and the nitrobenzene part cannot leave,so it cannot be lit.A pyridine group was introduced to increase the polarity of the tetraphenylethene core.However,because the presence of pyridine salt affects the quenching effect,the Probe-2 itself has a strong fluorescence,and the fluorescence intensity does not change much after the enzyme acts.Therefore,we introduced a nitrophenyl group on the core of tetraphenylethene to synthesize the probe Probe-3.Probe-3,a fluorescent probe,shows light-off fluorescence when detectingβ-glucuronidase.We continued to examine the optimal p H before and after incubation of fluorescent probes and enzymes,the most suitable time for incubation of fluorescent probes and enzymes,and the effects of different concentrations of enzymes,fluorescent probes,and sodium hydroxide on the effect.Finally,we also explored cis/trans configurations of fluorescent probes are used to detect the difference of β-glucuronidase.In summary,Probe-3 is a light-off fluorescent probe that can be used to detectβ-glucuronidase. |
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