Different Expression Patterns And Potential Roles Of Dip2a

Disconnected(disco)-interacting protein 2 homolog A(DIP2A)is a member of DIP2 protein family encoded by Dip2 a gene.In vitro based experiments indicated that Dip2 a expression occurs widely in mouse.However,expression and potential function of Dip2 a in tissues during embryonic development and postnatal life have not been well studied.Here,we have used a reporter mice generated in our laboratory in which Lac Z expression is driven by Dip2 a gene promoter and characterized Dip2a-Lac Z expression in embryos and adult tissues.Often,methods and techniques employed to investigate gene expression pattern are diverse,we describe X-Gal histochemistry as the only reliable method to identify Dip2 a gene expression despite of its disadvantages.In situ detection of DIP2 A protein by immunohistochemistry is tedious due to lack of validated antibodies.In particular,this has hampered understanding of Dip2 a expression pattern in some tissues,where IHC is technically challenging.Therefore,we report expression pattern of Dip2 a gene using a gene targeted mouse Dip2a-Lac Z which is a combined knockout and reporter allele.Interestingly,this mouse can be used to identify cells carrying as well as to map Dip2 a gene and hence endogenous Dip2 a gene expression pattern.We evaluated Dip2a-Lac Z allele as a reporter for endogenous Dip2 a gene expression pattern in mouse embryos and adults.In conclusion expression pattern of Dip2a-Lac Z recapitulates known expression patterns of Dip2 a gene in vivo where it is mainly localized to various epithelial,endothelial and some neuronal structures.We also report on novel embryonic and adult Dip2a-Lac Z expression patterns in mouse and discuss their implications for Dip2 a gene physiological and anatomical potential roles.