Detection Of Tumor Cells Using Tilted Fiber Grating Sensors With Sieving Halloysite Nanotube Coatings

Cancer is one of the leading causes of death worldwide.In recent years,cancer-related global mortality rates have been falling in developing countries,thanks to improvements in diagnosis and treatment.However,research into cancers such as stomach,liver,breast and lung is slow and difficult to detect at an early stage.Therefore,in the face of high medical costs and an increasing number of new cases,early diagnosis of cancer is a key factor.Optical fiber biosensors can be used as a cost-effective and relatively simple-to-implement alternative to well established bulky optical configurations for high sensitivity biological sample measurements.The miniaturized size and remote operation ability offer them a multitude of opportunities for single-point sensing in hard-to-reach spaces,even possibly in vivo.Based on the above research background,this paper mainly carries out the following work:(1)An optical fiber biomedical sensor modified by Halloysite Nanotubes is proposed.By self-assembly method,surface in engraved with Tilted Fiber Bragg Grating(Tilted Fiber Bragg Grating,TFBG)of the single-mode fiber surface modification Halloysite Nanotubes(HNTs)coating to enhance capturing the tumor cells.Because of the large surface area to volume ratio and the special structure of the submicron scale of the HNTs,they can provide an efficient binding capacity of tumor cells in a complex environment,while exerting less force on normal cells,so the fiber optic sensor modified by HNTs has the ability to capture tumor cells.(2)The detection of tumor cells by the optical fiber sensor was completed to achieve high precision and repeatable measurement.Design set the detection device based on micro circulation of tumor cells,and to normal cells and tumor cells were detected respectively,sensor detection time for 30 minutes,the linear response range is close to five orders of magnitude(10 to 10~5cells/m L),detection limit of 10 cells/m L,many times the repeatability of test validate the stability of the sensor.In addition,the fiber optic sensor is integrated with the microfluidic channel to achieve the accurate measurement of the sub-microliter capacity sample,and the temperature crossover problem in the measurement process is eliminated by the TFBG fiber core mode.This fiber-optic detection method provides a rapid and accurate detection method for the detection of trace biological samples for tumor cells,which is expected to be used in the early diagnosis and treatment evaluation of related diseases.