| Objective: Depression has become a global problem threatening human health due to its high incidence,great harm and easy recurrence.Anti-depressant drug therapy has some disadvantages,while exercise therapy has become an effective way of anti-depressant due to its unique advantages.Previous studies on depression have focused on the hippocampus,prefrontal cortex and hypothalamus,and whether the cerebellum,as an important part of the central system,is also related to the pathogenesis of depression has been rarely reported.This research USES the chronic unpredictable mild stimulation depression model rats(CUMS)depression model,in order to investigate the depression of aerobic exercise during the process of building or depression after the building of aerobic exercise intervention methods of spatial learning and memory ability of rats and rat cerebellum alkaline fibroblast growth factor(FGF-2)expression.Methods: Choose,SPF 3 months only 48 male SD rats,according to the 12 / group were randomly divided into four categories: natural control group(group C: 8 weeks natural feeding),CUMS depression model group(group D: 4 weeks ago CUMS natural feeding)4 weeks after building +,CUMS build mould during exercise group(E1 group: the first 4 weeks CUMS mold making and 4 weeks after aerobic exercise at the same time + natural feeding)and CUMS movement before and after the building group(E2 groups: the first four weeks CUMS 4 weeks after building + aerobic exercise).After the modeling of CUMS depression,behavioral indicators(weight change in rats,glucose consumption experiment,open box experiment)and physiological and biochemical indicators(whole-brain noradrenaline content was determined by ELISA)were used to evaluate whether the model of CUMS stress depression was successfully established.Morris water maze experiment was used to evaluate the spatial learning and memory ability of different groups of rats,so as to explore the influence of aerobic exercise during depression modeling or aerobic exercise after depression modeling on the spatial learning and memory ability of rats.After the end of all in vivo experiments,severed head samples were taken from the rats and cerebellum was isolated.Real-time PCR and Western-bolting were used to detect the expression of FGF-2 mRNA gene and FGF-2 protein in the cerebellum of the rats in each group.Results: 1.Modeling results of depression in CUMS:(1)General state: the rats in group C were in good mental state,eating normally and having soft and bright hair.The food intake of rats in group D decreased and their body shape became thinner.Rats in Group E1 ate normally and their fur was bright.During the modeling period of CUMS in the E2 group,the rats in CUMS were in poor condition and their body shape became thinner,but the condition was improved after aerobic exercise intervention.(2)Body weight: From the 7th day of modeling,there were significant differences in body weight of rats in each group;there were significant differences in body weight of rats in group D and E2 compared with group C(P < 0.05);there were significant differences in body weight of rats in group E1 compared with group C(P < 0.01).From the 14 th day of modeling to the end,compared with group C,there were significant differences in the weight of rats in group D,E1 and E2(P < 0.01).(3)Open box experiment: The OFT activity,OFT average speed,OFT central area distance,OFT central area time and OFT total distance in Group D were significantly lower than those in group C(P < 0.05);The relevant indexes of open box experiment in group E1 and Group E2 were significantly higher than that in group D(P < 0.05).However,there was no significant difference between group E1,E2 and C(P > 0.05).(4)sugar water consumption experiment: there was no significant difference in total water intake between the groups(P > 0.05).The white water consumption of rats in group D and E2 was significantly higher than that in group C(P < 0.01),and the white water consumption of rats in group D and E2 was significantly higher than that in group E1(P < 0.01).There were significant differences in white water consumption between Group E1 and Group E2(P < 0.01),while there were significant differences in sugar water consumption between group E1 and Group E2(P < 0.05).The consumption of sugar water in group D was significantly lower than that in group C(P < 0.01),and that in group E2 was significantly lower than that in group C(P < 0.05).The sugar water consumption ratio in group D and E2 was significantly lower than that in group C(P < 0.01),and the sugar water consumption ratio in group E1 was significantly higher than that in group E2(P < 0.01).(5)detection of whole-brain NE content in rats: the content of whole-brain NE in group D was significantly lower than that in group C(P < 0.01);the content of whole-brain NE in group E2 was significantly lower than that in group C(P < 0.05);the content of whole-brain NE in group E1 and E2 showed no significant difference(P > 0.05).2.Morris water maze test of spatial learning and memory ability of rats:(1)navigation experiment: groups of rats from 3 to 6 days end of the navigation experiment escape latency when appear difference and stable gradually,in group D rats escape the incubation period is significantly more than group C,E1 and E2 group(P < 0.05),while the E1 and E2 group escape latency in the process of the whole navigation experiment takes on no showed significant difference(P > 0.05).(2)space exploration experiment: the number of times the rats in group D crossed the platform was significantly lower than that in group C,E1 and E2(P < 0.01),while the number of times the rats in group E1 and E2 showed no significant difference(P > 0.05).3.Molecular biological detection:(1)Real-time PCR detection of FGF-2 mRNA expression: FgF-2 mRNA expression in group D was significantly lower than that in group C(P < 0.01),and FGF-2 mRNA expression in group E1 and E2 were significantly higher than that in group D(P < 0.01).(2)Expression of FGF-2 in the cerebellum of rats was detected by Western Blotting: The expression of FGF-2 in group D was significantly lower than that in group C(P < 0.01).The protein expression level of Group E1 was significantly higher than that of group D(P < 0.05),and that of group E2 was significantly higher than that of group D(P < 0.01).Conclusion: 1.Four weeks of CUMS stimulation can reduce the body weight,sugar water consumption,percentage of sugar water consumption,activity and the content of NE in brain tissue,and make the rats show depressive symptoms.This depressive symptom can lead to the down-regulation of the cerebellar fibroblast growth factor FGF-2 gene and protein expression in rats,which further leads to the impairment of the rats’ spatial learning and memory ability.2.Aerobic exercise can increase the consumption of sugar and water,maintain the content of brain tissue NE and behavioral activity at a certain level in rats in the CUMS depression model,and effectively improve the depressive symptoms caused by CUMS stimulation;After aerobic exercise,the expression of cerebellar fibroblast growth factor FGF-2 gene and protein were significantly up-regulated,thus maintaining the spatial learning and memory ability of rats.3.There was no significant difference between aerobic exercise intervention during the stimulation of CUMS and aerobic exercise intervention after the stimulation of CUMS in rats.These two different periods of aerobic exercise intervention have positive effects on cerebellar neurons,but the reason for the difference between the two groups may be the short modeling time,which can be further studied in the future. |
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